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Books > Science & Mathematics > Biology, life sciences > Molecular biology
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Tau Protein - Methods and Protocols (Hardcover, 1st ed. 2017)
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Tau Protein - Methods and Protocols (Hardcover, 1st ed. 2017)
Series: Methods in Molecular Biology, 1523
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This detailed volume gathers basic and advanced methods and
protocols from in vitro assays and in vivo models to address the
molecular and functional aspects of tau physiopathology. Divided
into five parts that illustrate the underlying molecular mechanisms
of Tau functions and dysfunctions in Alzheimer's disease and other
dementia referred to as tauopathies, this volume discusses timely
topics such as: conformational studies of native tau protein and
investigation of its physiological function in microtubules binding
and tubulin polymerization; in vitro methods of formation and
detection of tau oligomers and PHF-like fibrils; characterization
and in vitro introduction of post-translational modifications on
tau proteins for further functional studies; analytical tools for
the detection of tau proteins, their modifications and cellular
interactions, and MAPT gene mutations in various biological
samples; and cellular and in vivo models for the investigations of
tau physiopathology. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Practical
and cutting-edge, Tau Protein: Methods and Protocols is a valuable
resource that addresses the most recent aspects of Alzheimer's
disease research related to Tau protein and state-of-the-art
experimental techniques. It targets a broad scientific audience
including molecular biologists and biochemists, as well as all
researchers interested in exploring the functions of tau proteins.
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