In vitro propagation of virus free clones through meristem and
subsequent field evaluation of microclones were explored. The
present study also includes callus induction from leaf explant
followed by somatic embryogenesis, plant regeneration from somatic
embryos and subsequent field evaluation of embryo derived plants
for exploring possible occurrence of somaclonal variations. The
meristem culture, somatic embryos and bulblets derived plantlets
after proper acclimatization were transplanted to field and were
grown to maturity along with conventionally grown clove derived
plants as control. The plants were assessed for the occurrence of
somaclonal variation using different morphological and yield
related characters. The results illustrated in the present study
elucidated that meristem culture used for the micropropagation of
garlic could be effective in minimizing the incidence of somaclonal
variation and could be used for the production of virus free
planting materials. In addition, the high incidence of somaclonal
variations generated through somatic embryogenesis may be useful as
an alternative breeding tool for widening genetic basis for the
improvement of new garlic cultivars.
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