Fed-batch Fermentation is primarily a practical guide for
recombinant protein production in E. coli using a Fed-batch
Fermentation process. Ideal users of this guide are teaching labs
and R&D labs that need a quick and reproducible process for
recombinant protein production. It may also be used as a template
for the production of recombinant protein product for use in
clinical trials. The guide highlights a method whereby a medium
cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation
process can be accomplished within a single day with minimal
supervision. This process can also be done on a small (2L) scale
that is scalable to 30L or more. All reagents (media, carbon
source, plasmid vector and host cell) used are widely available and
are relatively inexpensive. This method has been used to produce
three different protein products following cGMP guidelines for
Phase I clinical studies.
General
| Imprint: |
Woodhead Publishing Ltd
|
| Country of origin: |
United Kingdom |
| Series: |
Woodhead Publishing Series in Biomedicine |
| Release date: |
September 2014 |
| First published: |
2015 |
| Authors: |
G. G. Moulton
|
| Dimensions: |
233 x 156 x 16mm (L x W x T) |
| Format: |
Hardcover
|
| Pages: |
188 |
| ISBN-13: |
978-1-907568-92-3 |
| Categories: |
Books
|
| LSN: |
1-907568-92-1 |
| Barcode: |
9781907568923 |
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