The advantages of obtaining a completely defined environment for
the growth of cells in vitro were recognized very early in the
history of cell culture (Lewis and Lewis, 1911). Continued interest
in the nutritional requirements of cells in vitro and in providing
an optimal environment for cells led to the development of the
complex nutrient mixtures available today in many media (Waymouth,
1972; Ham, 1965). However, serum remained an essential component of
medium for the growth of most cell types in culture. The question
of what factor (or factors) in serum was essential for cell growth
and survival remained unanswered for several decades. Initially,
experiments were designed to purify the "active component" of serum
for the growth of cells in culture. These experiments identified
fetuin (Fisher et at., 1958) and nonsuppressible insulinlike
activity (Temin et at., 1972) as important components of serum.
However, the complexity of serum and the very low levels of active
components in serum hindered progress in identi fying and isolating
serum factors."
General
| Imprint: |
Springer-Verlag New York
|
| Country of origin: |
United States |
| Release date: |
2012 |
| First published: |
1984 |
| Editors: |
Jennie Mather
|
| Dimensions: |
229 x 152 x 16mm (L x W x T) |
| Format: |
Paperback
|
| Pages: |
284 |
| Edition: |
Softcover reprint of the original 1st ed. 1984 |
| ISBN-13: |
978-1-4615-9363-8 |
| Categories: |
Books >
Medicine >
Pre-clinical medicine: basic sciences >
Anatomy >
General
|
| LSN: |
1-4615-9363-8 |
| Barcode: |
9781461593638 |
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