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Mitochondrial DNA - Methods and Protocols (Hardcover, 3rd ed. 2016)
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Mitochondrial DNA - Methods and Protocols (Hardcover, 3rd ed. 2016)
Series: Methods in Molecular Biology, 1351
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This third edition is comprised of well-established protocols that
are considered the gold standard in the field as well as new
methodologies for mitochondrial DNA analysis. of Mitochondrial DNA:
Methods and Protocols describes protocols for detecting mutations
in mitochondrial DNA, techniques to assess mitochondrial DNA
damage, visualization of mitochondrial DNA in situ, detection of
mitochondrial DNA nucleoids within the mitochondria, methods for
analyzing mitochondrial DNA replication, mitochondrial DNA-encoded
protein translation and mitochondrial DNA copy number, the latest
technologies for modifying the mitochondrial genome and methods for
the purification of proteins involved in the replication and
transcription of mitochondrial DNA. Written for the Methods in
Molecular Biology series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols and tips on
troubleshooting and avoiding known pitfalls. Authoritative and
practical, Mitochondrial DNA: Methods and Protocols, Third Edition
will be useful not only for mitochondrial researchers but also for
scientists studying human diseases where mitochondrial DNA
variation has been recognized as an important pathogenic factor,
including cancer and neurodegeneration.
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