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Directed Enzyme Evolution: Advances and Applications (Hardcover, 1st ed. 2017)
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Directed Enzyme Evolution: Advances and Applications (Hardcover, 1st ed. 2017)
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This book focuses on some of the most significant advances in
enzyme engineering that have been achieved through directed
evolution and hybrid approaches. On the 25th anniversary of the
discovery of directed evolution, this volume is a tribute to the
pioneers of this thrilling research field, and at the same time
provides a comprehensive overview of current research and the state
of the art. Directed molecular evolution has become the most
reliable and robust method to tailor enzymes, metabolic pathways or
even whole microorganisms with improved traits. By mirroring the
Darwinian algorithm of natural selection on a laboratory scale, new
biomolecules of invaluable biotechnological interest can now be
engineered in a manner that surpasses the boundaries of nature. The
volume is divided into two sections, the first of which provides an
update on recent successful cases of enzyme ensembles from
different areas of the biotechnological spectrum, including
tryptophan synthases, unspecific peroxygenases, phytases,
therapeutic enzymes, stereoselective enzymes and CO2-fixing
enzymes. This section also provides information on the directed
evolution of whole cells. The second section of the book summarizes
a variety of the most applicable methods for library creation,
together with the future trends aimed at bringing together directed
evolution and in silico/computational enzyme design and ancestral
resurrection.
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