This second volume of Research Methods in Neurochemistry develops
themes featured in the first volume and also introduces new
approaches to neurochemical methodology. A further chapter on cell
separation tech- niques seemed justified in view of its general
importance to the study of neurone-glia relationships and the
complexity of the methodological prob- lems involved. Biogenic
amines also receive more attention in chapters on the assay of
monoamine oxidase, catechol-o-methyltransferase and pineal indoles;
subcellular fractionation features again in chapters on methods for
the isolation and study of brain mitochondria and synaptic
vesicles. Among the new subjects dealt with in this volume, the
chapter on cell culture per- haps represents the most distinctive
departure from the themes of the first volume. Culture of neural
tissue and cells has, of course, been studied by neurobiologists
for several decades, but it is only recently that the neuro-
chemical potential of this approach has begun to be exploited. We
believe that neurochemists wishing to enter this field will find
Dr. Herschman's extensive treatment of the subject in this volume
of particular interest. Readers familiar with Volume 1 will note
that the balance of subjects in this volume is broadly similar.
Thus it has proved convenient to group the chapters under the same
section headings as before, with the inclusion of chapters on amino
acids in Section IV.
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