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Time-Resolved Mass Spectrometry - From Concept to Applications (Hardcover)
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Time-Resolved Mass Spectrometry - From Concept to Applications (Hardcover)
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Time is an important factor in physical and natural sciences. It
characterizes the progress of chemical and biochemical processes.
Mass spectrometry provides the means to study molecular structures
by detecting gas-phase ions with the unique mass-to-charge ratios.
Time-resolved mass spectrometry (TRMS) allows one to differentiate
between chemical states that can be observed sequentially at
different time points. Real-time mass spectrometric monitoring
enables recording data continuously with a specified temporal
resolution. The TRMS approaches introduced during the past few
decades have shown temporal resolutions ranging from hours down to
microseconds and beyond. This text covers the key aspects of TRMS.
It introduces ion sources, mass analyzers, and interfaces utilized
in time-resolved measurements; discusses the influence of data
acquisition and treatment; finally, it reviews most prominent
applications of TRMS in the studies of reaction kinetics and
mechanism, physicochemical phenomena, protein structure dynamics,
biocatalysis, and metabolic profiling. It will assist science and
engineering students to gain a basic understanding of the TRMS
concept, and to recognize its usefulness. In addition, it may
benefit scientists who conduct molecular studies in the areas of
chemistry, physics and biology.
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