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Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
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DNA Cloning: A Hands-on Approach (Hardcover, 1st ed. 2019)
Loot Price: R4,373
Discovery Miles 43 730
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DNA Cloning: A Hands-on Approach (Hardcover, 1st ed. 2019)
Expected to ship within 10 - 15 working days
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This book offers step-by-step instruction on DNA cloning, defined
as moving genes around plasmids, mutating genes, or mining new
genes. The aim is to provide those new to the field with reliable
and up-to-date practical guidance while at the same time conveying
the scope for creativity. After a brief synopsis of the history of
cloning, the fundamentals and prerequisites are explained,
covering, for example, software, vectors commonly used in the lab,
appropriate choice of restriction endonucleases, the preparation of
agarose gels, competent cells, and LB agar plates, and procedures
to be followed upon receipt of new plasmids. The remainder of the
book is devoted to the clear description of methods and individual
steps in cloning. Guidance is provided on the cut and paste method,
DNA sequencing, direct sequencing, primer design, PCR-based gene
insertion and deletion, epitope tag insertion, the use of RACE
technology, BAC recombineering, and much, much more. Sources of
error and a variety of techniques that make life considerably
easier when cloning are also examined in detail.
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