Bacillus thuringiensis spore forming, Gram positive aerobic
bacteria whose spores consist of the crystal protein which are
found to have the insecticidal activity to the specific insect
orders. Bacillus thuringiensis are considered to be cosmopolitan in
nature and a great difference occurs between the two strains from
different habitat. The present study was conducted on the
collection of samples and solation of the B.t. from these samples.
These isolates were identified on the basis of microscopic
techniques and staining techniques. Culturing of these isolates was
made on the specific media such as T3. Culturing of these bacteria
was done to get enough amount of the B.t. spore cell. Rearing of
the Tribolium castaneum a flour beetle was done by understanding
the physiology of the beetles. Then biotoxic effect of these
bacteria was determined by the process of Biotoxicity assay of the
Bacillus thuringiensis spores against Triboium castaneum in glass
vials in triplicates. The seven day repeated observation of these
isolates was presented in the tabular form and LC50 is calculated
by the Probit analysis programme.
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