Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment > Microscopy
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Super-Resolution Microscopy - A Practical Guide (Paperback)
Loot Price: R2,823
Discovery Miles 28 230
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Super-Resolution Microscopy - A Practical Guide (Paperback)
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This unique book on super-resolution microscopy techniques presents
comparative, in-depth analyses of the strengths and weaknesses of
the individual approaches. It was written for non-experts who need
to understand the principles of super-resolution or who wish to use
recently commercialized instruments as well as for professionals
who plan to realize novel microscopic devices. Explaining the
practical requirements in terms of hardware, software and sample
preparation, the book offers a wealth of hands-on tips and
practical tricks to get a setup running, provides invaluable help
and support for successful data acquisition and specific advice in
the context of data analysis and visualization. Furthermore, it
addresses a wide array of transdisciplinary fields of applications.
The author begins by outlining the joint efforts that have led to
achieving super-resolution microscopy combining advances in
single-molecule photo-physics, fluorophore design and fluorescent
labeling, instrument design and software development. The following
chapters depict and compare current main standard techniques such
as structured illumination microscopy, single-molecule
localization, stimulated emission depletion microscopy and
multi-scale imaging including light-sheet and expansion microscopy.
For each individual approach the experimental setups are
introduced, the imaging protocols are provided and the various
applications illustrated. The book concludes with a discussion of
future challenges addressing issues of routine applications and
further commercialization of the available methods. Guiding users
in how to make choices for the design of their own experiments from
scratch to promising application, this one-stop resource is
intended for researchers in the applied sciences, from chemistry to
biology and medicine to physics and engineering.
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