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Bacterial Sensors - Synthetic Design and Application Principles (Paperback)
Loot Price: R995
Discovery Miles 9 950
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Bacterial Sensors - Synthetic Design and Application Principles (Paperback)
Series: Synthesis Lectures on Synthetic Biology
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Bacterial reporters are live, genetically engineered cells with
promising application in bioanalytics. They contain genetic
circuitry to produce a cellular sensing element, which detects the
target compound and relays the detection to specific synthesis of
so-called reporter proteins (the presence or activity of which is
easy to quantify). Bioassays with bacterial reporters are a useful
complement to chemical analytics because they measure biological
responses rather than total chemical concentrations. Simple
bacterial reporter assays may also replace more costly chemical
methods as a first line sample analysis technique. Recent promising
developments integrate bacterial reporter cells with microsystems
to produce bacterial biosensors.This lecture presents an in-depth
treatment of the synthetic biological design principles of
bacterial reporters, the engineering of which started as simple
recombinant DNA puzzles, but has now become a more rational
approach of choosing and combining sensing, controlling and
reporting DNA 'parts'. Several examples of existing bacterial
reporter designs and their genetic circuitry will be illustrated.
Besides the design principles, the lecture also focuses on the
application principles of bacterial reporter assays. A variety of
assay formats will be illustrated, and principles of quantification
will be dealt with. In addition to this discussion, substantial
reference material is supplied in various Annexes.Table of
Contents: Short History of the use of Bacteria for Biosensing and
Bioreporting / Genetic Engineering Concepts / Measuring with
Bioreporters / Epilogue
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