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Artificially Controllable Nanodevices Constructed by DNA Origami Technology - Photofunctionalization and Single-Molecule Analysis (Hardcover, 1st ed. 2015)
Loot Price: R2,859
Discovery Miles 28 590
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Artificially Controllable Nanodevices Constructed by DNA Origami Technology - Photofunctionalization and Single-Molecule Analysis (Hardcover, 1st ed. 2015)
Series: Springer Theses
Expected to ship within 10 - 15 working days
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In this book, the author deals mainly with two topics: (1)
single-molecule visualization of switching behaviors in the DNA
nanoframe system utilizing different kinds of molecular switches
through the use of high-speed atomic force microscope (AFM); (2)
construction of photocontrollable DNA nanostructures in programmed
patterns and direct visualization of the dynamic assembling
process. Here, high-speed AFM was employed to observe the dynamic
movements of single molecules. Compared to a traditional
single-molecule analysis method, such as fluorescence spectroscopy
or electron microscopy, high-speed AFM makes possible the real-time
observation of molecule behaviors. DNA nanostructures were designed
and assembled as scaffolds to incorporate interested biomolecules.
The observations were carried out under robust conditions without
complicated pretreatment. Moreover, the photoresponsive molecules
were successfully assembled into around 100 nm-sized DNA
nanostructures. The assembly/disassembly of nanostructures can be
regulated reversibly by photoirradiation. This book explains how
DNA origami has gradually become a useful tool for the
investigation of biochemical interactions in defined nanospace. It
also shows the possibility of DNA nanostructures acting as
nanodevices for application in biological systems, serving as a
good introduction to basic DNA nanotechnology.
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