This book constitutes the refereed proceedings of the First International Conference on Formal Methods in Macro-Biology, FMMB 2014, held in Noumea, New Caledonia, in September 2014. The 7 revised full and 3 short papers presented together with 7 invited presentations were carefully reviewed and selected from 17 submissions. The scientific program consists of papers on a wide variety of topics, including ecological systems, medical applications, logical frameworks, and discrete continuous and hybrid models for the analysis of biological systems at macroscopic levels.