This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field

The collection brings together texts of Brazilian researchers who are dedicated to themes related to studies of youth cultures: social interactions, subcultures, identities and belonging, pop culture, social movements, migration, consumption and materialities, generational exchanges, media representations and digital media, among others. The objective is to promote a broad dialogue that includes fields of knowledge such as communication and social sciences, as well as local perspectives that represent the huge and rich diversity of the Brazilian regions. At the same time, the book proposes to discuss the reflexivity of such local youth cultures in the face of a global context that challenges, with ruptures and permanencies, the very idea of youth. The book seeks to fill the gap of a selection of scientific texts by Brazilian authors, about Brazilian youth cultures, aimed at foreign researchers.

This open access book offers a comparative overview on Portuguese emigration in Europe and outside the EU in times of recession. It looks at Portuguese emigrants who, after the crisis of 2008, moved both intra-EU, such as UK, France, Switzerland, Germany and Spain, but also into countries with historical links, such as the USA and Canada, and to Portuguese speaking countries such as Brazil, Angola and Mozambique, as well as the processes of return. In addition to the dynamics of movement, the book provides an in-depth analysis of the heterogeneity of this emigration. It deepens the multifaceted identities concerning social and professional pathways among highly skilled and less skilled emigrants. The labour market continues to be the main regulatory force of Portuguese emigration, which helps to explain the outflow and the processes of settlement and return. Nonetheless, this book demonstrates that non-economic factors have likewise been of great importance in the decision to emigrate. As such this book will be a valuable read to policy makers, students and scholars in migration.

The collection brings together texts of Brazilian researchers who are dedicated to themes related to studies of youth cultures: social interactions, subcultures, identities and belonging, pop culture, social movements, migration, consumption and materialities, generational exchanges, media representations and digital media, among others. The objective is to promote a broad dialogue that includes fields of knowledge such as communication and social sciences, as well as local perspectives that represent the huge and rich diversity of the Brazilian regions. At the same time, the book proposes to discuss the reflexivity of such local youth cultures in the face of a global context that challenges, with ruptures and permanencies, the very idea of youth. The book seeks to fill the gap of a selection of scientific texts by Brazilian authors, about Brazilian youth cultures, aimed at foreign researchers.

This open access book offers a comparative overview on Portuguese emigration in Europe and outside the EU in times of recession. It looks at Portuguese emigrants who, after the crisis of 2008, moved both intra-EU, such as UK, France, Switzerland, Germany and Spain, but also into countries with historical links, such as the USA and Canada, and to Portuguese speaking countries such as Brazil, Angola and Mozambique, as well as the processes of return. In addition to the dynamics of movement, the book provides an in-depth analysis of the heterogeneity of this emigration. It deepens the multifaceted identities concerning social and professional pathways among highly skilled and less skilled emigrants. The labour market continues to be the main regulatory force of Portuguese emigration, which helps to explain the outflow and the processes of settlement and return. Nonetheless, this book demonstrates that non-economic factors have likewise been of great importance in the decision to emigrate. As such this book will be a valuable read to policy makers, students and scholars in migration.