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This volume contains the lectures given at the NATO Advanced Study
Institute on "Biophysics of Photoreceptors and Photomovements in
Microorganisms" held in Tir renia (Pisa), Italy, in September 1990.
The Institute was sponsored and mainly funded by the Scientific
Affairs Division of NATO; the Physical Science Committee and the
Institute of Biophysics of National Research Council of Italy also
supported the School and substantially contributed to its success.
It is our pleasant duty to thank these institu tions. Scientists
from very different backgrounds contributed to the understanding of
this fast developing field of research, which has seen considerable
progress during the last years. The areas of expertise ranged from
behavioral sciences, supported by sophi sticated techniques such as
image analysis or laser light scattering, to spectroscopy, ap
plied, in different time domains, to the study of the primary
photoreactions, to electro physiology, biochemistry or molecular
biology, with the aim of analyzing the various steps of the
transduction chains and how they control the motor apparatus of the
cells. The organisms studied covered a wide range, from bacteria to
algae, fungi and other eukaryotes. Thus, the ASI represented a
successful opportunity for carrying on and imple menting an
interdisciplinary approach to the study of the biophysical basis of
photore ception and photosensory transduction in aneural organisms,
with special attention to the basic phenomena and the underlying
molecular events. We hope that this book has caught the spirit in
which the ASI was conceived."
The main purpose of this book is to unify approaches and ideas in
the field of aneural sensory transduction. This field has recently
come to the attention of several research groups in various
disciplines, and their number seems to be growing. Unfortunately,
because of the diverse scientific backgrounds of the researchers in
the field, the apparent heterogeneity of experimental techniques
(i. e., behavioral response analysis, sophisticated biochemical and
genetic manipulations, conventional and pulsed laser spectroscopy)
and theoretical approaches may be discouraging, for both the
experienced worker and the new comer. Actually, this heterogeneity
is more apparent than real, and unifying concepts, approaches, and
ideas already exist, particularly with respect to all the questions
concerning the role of membranes and their properties (such as ion
permeability, electric potentials, and active transport) in the
various steps of sensory perception and transduction processes. It
is currently accepted that most, if not all, the fundamental facts
in molecular sensory physiology of aneural organisms, be they
chemosensory, photosensory, or geosensory, can ultimately be
understood in terms of a few basic ideas. Each chapter of this book
emphasizes and clarifies the role of mem brane properties and
phenomena in the particular sensory response examined. Of course,
in some cases, this task has been rather complex because of the
limited amount of experimental data clearly supporting a
membrane-based model of sensory transduction."
This book is based on the lectures given at the NATO Advanced Study
Institute on "Sensory Perception and Transduction in Aneural
Organisms" held in Volterra (Pisa. Italy) from the third to the
fourteenth of September. 1984. The Advanced Study Institute was
planned as a high level course dealing with several aspects and
problems of sensory perception and transduction of diverse
environmental stimuli in aneural organisms. Scientists from
different fields and cultural backgrounds were present at the
meeting. both as lecturers and as students. The lectures and the
discussions that followed represented a well integrated interdisci
plinary approach to the questions considered. At the end of the
Advanced Study Institute course. it was quite clear that. notwith
standing the apparent heterogeneity of the topics dealt with.
unifying concepts and ideas already existed, among the most
important being the role of membranes and their physicochemical
properties. All this should be reflected in the content of this
book. We gratefully acknowledge the financial sponsorship of the
Scientific Affairs Division of NATO (Brussels), that made both the
Advanced Study Institute on "Sensory Perception and Transduction in
Aneural Organisms" and this book possible. Finally. we are also
indebted to Ms. Pat Parham Morgan who expertly retyped all the
chapters of the book and Ms. Leslie Schmidt of Plenum Publishing
Co. provided us valuable advice and suggestions on the preparation
of this book. G. Colombetti F. Lenci P. S."
A NATO Advanced Study Institute on "Light as Energy Source and
Information Carrier in Plant Photo physiology" was held at
Volterra, Italy, from September 26 to October 6, 1994, in order to
consider the fundamental role that light plays in plant growth and
development. This book summarises the main lectures given at this
meeting which concentrated on both photochemical energy conversion
and signalling (photosensing) aspects. Light harvesting and
conversion into chemical energy in photosynthesis occurs at the
level of chlorophyll/carotenoid containing photosystems in plants.
Pigments are non covalently bound to a variety of polypeptides
which serve as a specific scaffolding, necessary to determine the
energy coupling between pigments and thus allowing rapid excitation
energy trasfer from the antenna to the special reaction centre
chlorophylls. Data from transient, time resolved spectroscopies, in
the femtosecond and picosecond domain, together with model
calculations, suggest that this process occurs in the 20-100
picosecond time span. The special ll u ture of reaction centre
complexes, ensures rapid primary charge separation, probably in the
order of 1-3 picoseconds, with subsequent charge stabilisation
reactions proceeding in the hundreds of picoseconds range. The
recently resolved crystallographic structure of LHCII, the
principal antenna complex of plants, allows precise determination
of pigment-pigment distances and thus permits calculation of
approximate chlorophyll-chlorophyll Forster hopping rates, which
are in good agreement with time resolved measurements."
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