Confusion. Lust. Fear. Passion. It started the moment she passed out in my arms. Seduction. Vile. Depraved. Desire. She couldn't remember her past but looked to me like I was her future. My walls were built. My heart was cold. My emotions vacant. After being alone for five years with only my motorcycle club at my side, she was who I had been searching for. The one I had needed all along to light up the darkness surrounding my life. Just when I thought I had her, just when I thought I could finally allow myself that happiness I didn't think I deserved. She was ripped from my hands...

Ruined. Tortured. Broken. What could have been the perfect childhood, turned into a hell that the devil himself would shy away from. I was forced to deal with an evil that would make the darkest of souls cringe. Lily Noel was scarred but beautiful. She was perfectly flawed and everything that I needed. Attraction bordered on obsession. Passion bordered on ecstasy. Addiction bordered on insanity. Nothing could get in the way of our love. Nothing... *This book contains dark themes including sexual assault and past child abuse*

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.