This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

As authors, we are convinced that the time has finally arrived in academe for an extensive, experience?based, firsthand, seamless examination of what we are calling crossover pedagogy. There is no book?length examination of facultystudent affairs administrators collaboration in the academic realm anywhere. Nobody has yet to produce a case?based, hands?on, book?length treatment of how (and why) faculty and student affairs administrators can co?teach, co?author, and co?consult with one another as co?equal educators and campus leaders-with each group complementing the other in terms of their special skills, knowledge, background, and experiences. Without coming to practical terms with the case for collaboration that the above authors make, the why rationale developed in these publications on the topic of faculty?administrator collaboration (sometimes referred to as "blended" efforts) around the teaching?learning venture is lost in the logistics of technical policy issues and challenges.

This book introduces various 3D printing systems, biomaterials, and cells for organ printing. In view of the latest applications of several 3D printing systems, their advantages and disadvantages are also discussed. A basic understanding of the entire spectrum of organ printing provides pragmatic insight into the mechanisms, methods, and applications of this discipline. Organ printing is being applied in the tissue engineering field with the purpose of developing tissue/organ constructs for the regeneration of both hard (bone, cartilage, osteochondral) and soft tissues (heart). There are other potential application areas including tissue/organ models, disease/cancer models, and models for physiology and pathology, where in vitro 3D multicellular structures developed by organ printing are valuable.

This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand. After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, RNA-Protein Complexes and Interactions: Methods and Protocols provides a set of useful protocols, both basic and advanced, designed to inspire researchers working with RNA and RNA-interacting proteins.

This book presents the proceedings of the 2nd Pacific Rim Statistical Conference for Production Engineering: Production Engineering, Big Data and Statistics, which took place at Seoul National University in Seoul, Korea in December, 2016. The papers included discuss a wide range of statistical challenges, methods and applications for big data in production engineering, and introduce recent advances in relevant statistical methods.

The book focuses on four broad topics related to trapping of agriculturally important tephritid fruit flies, namely i) lures and traps, ii) invasion biology and detection of infestations, iii) attract and kill systems, and iv) trade regulations and risk assessment. This comprehensive structure progresses from the biological interaction between insect and lures/traps to the area-wide use of trapping systems to the utilization and impact of trapping data on international trade. The chapters include accounts of earlier research but are not simply compendia and instead evaluate past and current work as a tool for critical analysis and proposal of productive avenues for future work. At present there is no book available that deals with fruit fly trapping in such a broad context. Our book fills this gap and serves as a global reference for both those interested in fruit flies specifically as well as anyone dealing with the threat of invasive agricultural insects in general.

This book examines epistemic pluralism, a brand new area of research in epistemology with dramatic implications for the discipline. Challenging traditional assumptions about the nature of justification, an expert team of contributors explores pluralism about justification, with compelling first-order results - including analysis of the various requisites one might want to impose on the notion of justification (and therefore of knowledge) and why. It is shown why a long-lasting dispute within epistemology about the nature of justification has reached a stalemate and how embracing a different overarching outlook might lead to progress and aid better appreciation of the relationship between the various epistemic projects scholars have been pursuing. With close connections to the idea of epistemic relativism, and with specific applications to various areas of contemporary epistemology (such as the debate over epistemic norms of action and assertion, epistemic peers' disagreement, self-knowledge and the status of philosophical disputes about ontology) this fascinating new volume is essential reading for scholars, researchers and advanced students in the discipline.

This book provides a systematic presentation of the principles and practices behind the synthesis and functionalization of graphene and grapheme oxide (GO), as well as the fabrication techniques for transparent conductors from these materials. Transparent conductors are used in a wide variety of photoelectronic and photovoltaic devices, such as liquid crystal displays (LCDs), solar cells, optical communication devices, and solid-state lighting. Thin films made from indium tin oxide (ITO) have thus far been the dominant source of transparent conductors, and now account for 50% of indium consumption. However, the price of Indium has increased 1000% in the last 10 years. Graphene, a two-dimensional monolayer of sp2-bonded carbon atoms, has attracted significant interest because of its unique transport properties. Because of their high optical transmittance and electrical conductivity, thin film electrodes made from graphene nanosheets have been considered an ideal candidate to replace expensive ITO films. Graphene for Transparent Conductors offers a systematic presentation of the principles, theories and technical practices behind the structure-property relationship of the thin films, which are the key to the successful development of high-performance transparent conductors. At the same time, the unique perspectives provided in the applications of graphene and GO as transparent conductors will serve as a general guide to the design and fabrication of thin film materials for specific applications.

This volume is about the social identities of young Indigenous people in contemporary Australia, based on fieldwork in the rural community of Yarrabah, in Queensland. This case study of Yarrabah is based on seventeen ethnographic interviews with women and men in their twenties. With the aim of exploring how diverse social discourses have influenced the social identities of young Indigenous people in contemporary Australia, this book represents the life histories of these young people in Yarrabah in the context of both the institutions with which they interact and the everyday shape of life in Yarrabah. This volume also provides new material for discussion of the ways in which Indigenous value systems, broadly understood by the participants to be based on collectivism, constantly come into conflict with Western values based on individualism. While the young Indigenous people of Yarrabah do continuously interact not only with multi-cultural Australia but also with global influences, they are constantly aware of their own distinctiveness in both contexts.

This book introduces readers to the concepts of sustainability and philosophy of slowness for the management of public entities such as cities or regions. While many urban communities face economic challenges that clearly show the limitations of growth and ever-increasing speed, this book explores an alternative, thought-provoking standpoint in five chapters. The first chapter explains the importance and essence of slowness, smallness and sustainability for public organizations, while the second addresses the concept of "slow life" in an emotional society. Chapter three examines the issue of "slow management" and presents arguments for the value of small businesses as the true foundation of the economy. Chapter four rounds out the coverage with a focus on agriculture. Finally, in chapter five, the authors discuss the overall benefits of a "slow and curvy" management style in order to provide happiness, economic and social sustainability.

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes. This is done while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

This book explores the 1907 Korean Revival Movement from a self psychological perspective. The examination of the psychological processes in the movement based on Heinz Kohut's self psychology can shed light on religious experiences as selfobject experiences by identifying the sense of defeatedness and helplessness that Korean people experienced under Japanese occupation as what Kohut calls self-fragmentation of the Korean group self and explaining its therapeutic functions which facilitate potential for the narcissistic nourishment of the fragmented group self leading to renewed self-esteem, transformation, and empowerment of the Korean people. Korean people in the early 1900s experienced abuses and oppression by corrupt officials and exploitation by Japanese government. Through religious experiences which emphasized the individual repentance, the experience of God through the spirit, emphasis on prayer, and eschatological faith, the Korean Revival Movement in 1907 enabled its followers to experience mirroring and idealizing selfobjects which function as a role of transforming the lower shape of narcissism into the higher one.

The book is the first attempt to offer a holistic and integrated exploration of the political-economic framework underpinning economic regionalism. In doing so it provides a much-needed contribution to the literature on international political economy, international relations and Asian political economy in relation to economic regionalism. The existing literature provides broad generalizations and limited discussion on economic integration (i.e. free trade agreements, FTA) with most analyses of regionalism generally contained to the field of economics with a focus on the welfare implications of FTAs, both for participating countries and the world as a whole. Readers of this book can view economic regionalism from a variety of perspectives with input from Chinese, Japanese and Korean research institutes, business and industry groups, and government officials. Drawing on the considerable country experience and expertise of the authors, the book attempts to unravel the paradox of the market-driven economic globalization process (regionalism) and address a serious gap in the current literature relating to the political-economic characteristics and strategies of China, Japan and Korea in relation to economic regionalism.

The proceedings from Parallel CFD 2006 covers all aspects of parallel computings and its applications. Although CFD is one of basic tools for design procedures to produce machineries, such as automobiles, ships, aircrafts, etc., large scale parallel computing has been realized very recently, especially for the manufactures. Various applications in many areas could be experienced including acoustics, weather prediction and ocean modeling, flow control, turbine flow, fluid-structure interaction, optimization, heat transfer, hydrodynamics.
- Report on current research in the field in an area which is rapidly changing
- Subject is important to all interested in solving large fluid dynamics problems
- Interdisciplinary activity. Contributions include scientists with a variety of backgrounds