This volume presents 12 comprehensive and timely review articles on some of the new tools and applications of biochemical engineering and biotechnology. The tools range from screening methods for novel biocatalysts and products, fluorescence spectroscopy and mass spectrometry for monitoring and analysis of cellular processes via mathematical models and protein expression systems for metabolic engineering to new bioreaction and separation devices. The applications cover the uses of animal and tissue cultures, insect cells, recombinant and marine microorganisms for the production of a variety of important bioproducts.

Ch. P. Kubicek, Vienna: "The Cellulase Proteins of Trichoderma reesei: Structure, Multiplicity, Mode of Action and Regulation of Formation" Dr.A. Singh, Dr. P.K.R. Kumar, Dr. K. Sch}gerl, Hannover: "Bioconversion of Cellulosic Materials to Ethanol by Filamentous Fungi" Dr. T. Coolbear, Palmerston North, New Zealand, Dr. R.M. Da- niel, Dr. H.W. Morgan, Hamilton, New Zealand: "The Enzymes from Extreme Thermophiles: Bacterial Sources, Thermostabilities and Industrial Relevance" Dr. N. Weber, M}nster, Dr. D. C. Taylor, Dr. E. W. Under- hill, Saskatoon (Canada): "Biosynthesis of Storage Lipids in Plant Cell and Embryo Cultures"

The state of the art of the bioengineering aspects of the morphology of microorganisms and their relationship to process performance are described in this volume. Materials and methods of the digital image analysis and mathematical modeling of hyphal elongation, branching and pellet formation as well as their application to various fungi and actinomycetes during the production of antibiotics and enzymes are presented.

History of Modern Biotechnology, devided into two volumes (69 and 70), is devoted to the developments in different countries. N.W.F. Kossen: The Morphology of Filamentous Fungi.- H. Bockeer, W.A. Knorre: Antibiotica Research in Jena from Penicillin and Nourseothricin to Interferon.- K. Schugerl: Development of Bioreaction Engineering.- R. Katzen, G.T. Tsao: A View of the History of Biochemical Engineering.- J. M. Woodley: Selected Advances in Enzyme Technology.- H.R. Bungay: Computer Applications in Bioprocessing.- W. Beyeler, E. DaPra, K. Schneider: Automation of Industrial Bioprocesses.-"

Alongside presenting the fundamentals, this book reviews the state of the art of mathematical modeling and control of bioprocesses, while demonstrating the application in various biological systems important to industry. At the same time, the application of different types of models and control strategies are illustrated, taking into account the recent developments in reactor modeling. In addition to modeling and control, the metabolic flux analysis and the metabolic design and their application to bioprocesses are considered.

This special volume "Tools and Applications of Biochemical Engineering Science" is dedicated to Professor Wolf-Dieter Deckwer on the occasion of his 60th bir- day. It was a great pleasure for me to act together with Professor Karl Schtigerl as volume editor and to present here a collection of 11 outstanding review articles written mainly by former students, associates, colleagues and friends of Wolf- Dieter Deckwer. The title of this special volume well reflects the research interests and sci- tific pursuit of Wolf-Dieter Deckwer during his more than 20 years' work in the area of biochemical engineering, particularly during the last 15 years when he was the head of the Biochemical Engineering Division of GBF (German Nat- nal Research Center for Biotechnology). He has decisively pushed the devel- ment not only of "software tools" ranging from analytical means and mathe- tical models for monitoring and understanding cellular processes to gene expression systems for designing microorganisms, but also of "hardware tools" such as computer control systems, bioreaction and separation devices for eff- tively producing a variety of bioproducts on semi-production scale. New developments in some of these important tools in biochemical engineering are reviewed in articles included in this volume. Wolf-Dieter Deckwer was among the leading biochemical engineers who timely pointed out the necessity of applying these tools in an integrated manner for bioprocess development. By establishing "Integrated Bioprocess Development" as one of the GBF main - search topics as early as 1990 he also actively promoted this idea.

Die prazise UEberwachung von Bioprozessen ist eine der wichtigsten Voraussetzungen fur ihre Systemanalyse, mathematische Modellierung, Regelung und Dokumentation. Die Umsetzung von Ausgangsprodukten mit Hilfe von Biokatalysatoren muss genau verfolgt werden, um hohe Produktivitat und Produktqualitat zu erreichen. Dieses Buch fasst erstmals die allgemeinen und speziellen Messmethoden, die fur die Prozessuberwachung von zentraler Bedeutung sind, zusammen. Das Spektrum der vorgestellten Methoden umfasst verschiedene in-situ Techniken fur die UEberwachung von Zustand- und Kontrollvariablen, sterile on-line-Probeentnahmetechniken, Probekonditionierung, sowie moderne on-line- und die wichtigsten off-line-Analysemethoden fur die Prozessuberwachung unter Berucksichtigung der neuesten Entwicklungen auf diesem Gebiet. Die Anwendungen dieser Methoden werden an typischen Bioprozessen beispielhaft aufgezeigt. Daruber hinaus werden Beispiele fur die anaerobe Abwasserbehandlung beschrieben und die wirtschaftlichen, oekologischen und Sicherheitsaspekte dieser Prozesse berucksichtigt.

Volume 3 of Bioreaction Engineering covers the general principles and techniques of bioprocess monitoring and their application for various bioprocesses. Methods based on the author’s long standing experience working with various bioprocesses are applied within the book. In particular, the cultivation of Baker’s yeast; production of fusion protein with recombinant E. Coli, alkaline serine protease production with Bacillus licheniformis; production of penicillin V with Penicillin chrysogenum; Cephalosporin C with Acremonium chrysogenum and tetracycline with Streptomyces aureofaciens are considered. This book deals with the monitoring of batch and perfusion cultivations of animal cells and production of monoclonal antibodies with hybridoma cells, Antithrombin III with BHK and CHO cells and ß -galactosidase with insect cells. The topics covered include: Bioprocess monitoring techniques Cultivation of Saccharomyces cerevisiae Production of Fusion Protein with Recombinanat E. coli Alkaline Protease Production with Bacillus licheniformis Antibiotica Production by Fungi and Streptomycetes Continuous Production of Primary Metabolites with Suspended and Immobilized Microorganisms Cultivation of Animal Cells and Production of Proteins Anaerobic Waste Water Treatment Fast Process Monitoring Techniques Image Analysis of Cells and Cell Aggregates Evaluation of Experimental Data to the Calculation of Metabolite Flux in Microorganisms and Animal Cells Signal Evaluation, Automation and Expert Systems for Process Monitoring Bioprocess Monitoring is invaluable for process engineers, analytical chemists and researchers in biotechnological, pharmaceutical, environmnental and chemical industries.