This study was carried out at the Department of Microbiology and Biotechnology Karnatak University, Dharwad. The bacterial strains were collected from various clinical samples like urine, infected blood, pus, wound swabs, catheters and cerebrospinal fluid (CSF) from different inpatients of Karnatak Institute of Medical sciences (KIMS), Civil Hospital, and local hospitals and Nursing Homes of Hubli-Dharwad. One thousand fifty individuals were included in the study, seven hundred and fifty males, and three hundred females.For isolation and identification of Staphylococcus aureus the samples were inoculated on nutrient agar and selective medium, mannitol salt agar (MSA). The characteristic colonies producing golden yellow or cream, round, raised regular colonies on nutrient agar and yellow coloration around MSA were presumptively selected as total of 675 Staphylococcus spp. isolates were procured. These isolates were subjected for cultural, microscopical and biochemical analysis for confirmation

A disease is an abnormal condition affecting the body of an organism. It may be caused by external factors, such as infectious disease, or it may be caused by internal dysfunctions, such as autoimmune diseases.The diabetes nowadays causing more number of deaths and creating more problems irrespctive age. This can be controlled with proper diet and exercise that enhances or maintains physical fitness and overall health and wellness.The traditional system of medicine has given us those plants that can be used to control and cure many diseases including diabetes. Indian system of medicine has given more information about medicinal plants and its uses. In present study Nymphaea odorata was used, which was collected from Dandeli forest area of karnataka, India. Ayurveda, sidda medicine, unani, homeopathy has revealed the some causes and given some remedies for diabetes.The present study acknowledges Department of Biotechnology, New Delhi for financial support under DBT-IPLS, PURSE program. Department of Biotechnolgy, Dept of Zoology, Karnatak University, Dharwad.I express my sincer gratitude to lambert academic publshing house, Ge