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Vinca rosea L. Syn. Catharanthus roseus L. G. Don. is an important
medicinal plant which belongs to the family Apocynaceae. The
present study deals with Micropropagation, Callogenesis, Cell
suspension culture & Antimicrobial activity of Vinca rosea L..
Explants were cultured on MS basal medium and supplemented with
different concentrations of BAP (mg/l) and NAA (mg/l) for
micropropagation whereas 2, 4-D (mg/l) and Kin (mg/l) for
callogenesis. Multiple shoots were obtained on all the
concentrations of BAP and NAA, but BAP (1 mg/l) showed best
response (90% & 80%).Similarly best callus response was
observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in
all the explants (95% from leaf, 80% from node & 60% from
fruit). Cell suspension culture have also been established in the
present study according to which the calluses gave suspensions
consisting of both the embryogenic and non-embryogenic cluster of
the cells in 2, 4- D + Kin (1+1 mg/l) and gave best response
(70%).The present study also describes the comparison of in vivo
and in vitro antimicrobial activity of Vinca rosea. Three different
pathogenic bacterial strains .) and three different fungal strains
were used.
The given plant, Solanum tuberosum was grown in the field as well
as in petri plants by seeds.The different explants such as leaf,
node, internodes, bud and roots were taken for micropropagation.
These explants were grown in vitro after thorough sterilization
using different PGR in different media composition.The PGR used
were 2,4-D, BAP and NAA. Their combination were also used i.e.
2,4-D+BAP, BAP+NAA and 2,4-D+NAA in different concentration.The
most effective and suitable media composition was MS (Murashige
& Skoog, 1962) basal medium with BAP (2.0 mg/l) for
regeneration from nodal explant of Solanum tuberosum.The optimum
temperature was found to be 23 02 oC. The micropropagation occurred
more precisely at about 30 (g/l) sucrose concentrations. The most
effective photoperiod for the regeneration of Solanum tuberosum was
16 hours light period. The pH 5.7 was considered to be the best for
the micropropagation of Solanum tuberosum.
Solanum nigrum was initially grown, in the soil through seeds, and
also in the petri dishes. For the purpose of micropropagation, MS
basal medium was used along with different PGRs i.e, BAP, 2,4-D,
NAA and their different combinations. Effects of different physical
and chemical factors on the in vitro growth of these explants were
studied. As far as, physical factors were concerned, it was noted
that 22 2 C for in vitro growth of Solanum nigrum was used. It was
also observed that the micropropagation of Solanum nigrum in liquid
medium gave only 5% in vitro growth. Whereas, solidified medium
(using Difco-Bacto agar as Solidifying agent) gave 80% in vitro
growth for the plant. The effect of different photoperiods was also
observed with 16hrs photoperiod (3000 lux) in in vitro
micropropagation of Solanum nigrum. Suitable maximum pH value for
micropropagation was found to be 5.7 for in vitro growth was noted
for Solanum nigrum.
The present study deals with the callogenesis and micropropagation
of grapes (Vitis vinifera L.) and biochemical investigation of
protein content and peroxidase activity during different stages of
growth. In the protocol of micropropagation, four different types
of media with different concentration of auxin, cytokinin and their
combinations were used. Best result was obtained with the TDZ 25.0
(uM) with 90% frequency rate. In the induction of callogenesis
procedure, three different types of media concentration of auxin,
cytokinin and their combination were used with leaf and node as
explant. The best result observed from leaf explant obtained from
2,4-D 3.0 (mg/l) with maximum 90% frequency rate. Best result for
callus formation from nodal explant obtained from 2,4-D 3.0 + BAP
1.0 + 1.0 TDZ (mg/l) with 90% frequency rate.In the estimation of
soluble protein content best result of in vivo grown cultures
obtained from leaf explant (0.085 0.643) in 3rd subculture. In the
comparison of specific activity of peroxidase enzyme, best result
of in vivo grow cultures obtained from shoot tip explant (0.00063
0.692) in 3rd subculture.
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