Vinca rosea L. Syn. Catharanthus roseus L. G. Don. is an important medicinal plant which belongs to the family Apocynaceae. The present study deals with Micropropagation, Callogenesis, Cell suspension culture & Antimicrobial activity of Vinca rosea L.. Explants were cultured on MS basal medium and supplemented with different concentrations of BAP (mg/l) and NAA (mg/l) for micropropagation whereas 2, 4-D (mg/l) and Kin (mg/l) for callogenesis. Multiple shoots were obtained on all the concentrations of BAP and NAA, but BAP (1 mg/l) showed best response (90% & 80%).Similarly best callus response was observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in all the explants (95% from leaf, 80% from node & 60% from fruit). Cell suspension culture have also been established in the present study according to which the calluses gave suspensions consisting of both the embryogenic and non-embryogenic cluster of the cells in 2, 4- D + Kin (1+1 mg/l) and gave best response (70%).The present study also describes the comparison of in vivo and in vitro antimicrobial activity of Vinca rosea. Three different pathogenic bacterial strains .) and three different fungal strains were used.

The given plant, Solanum tuberosum was grown in the field as well as in petri plants by seeds.The different explants such as leaf, node, internodes, bud and roots were taken for micropropagation. These explants were grown in vitro after thorough sterilization using different PGR in different media composition.The PGR used were 2,4-D, BAP and NAA. Their combination were also used i.e. 2,4-D+BAP, BAP+NAA and 2,4-D+NAA in different concentration.The most effective and suitable media composition was MS (Murashige & Skoog, 1962) basal medium with BAP (2.0 mg/l) for regeneration from nodal explant of Solanum tuberosum.The optimum temperature was found to be 23 02 oC. The micropropagation occurred more precisely at about 30 (g/l) sucrose concentrations. The most effective photoperiod for the regeneration of Solanum tuberosum was 16 hours light period. The pH 5.7 was considered to be the best for the micropropagation of Solanum tuberosum.

Solanum nigrum was initially grown, in the soil through seeds, and also in the petri dishes. For the purpose of micropropagation, MS basal medium was used along with different PGRs i.e, BAP, 2,4-D, NAA and their different combinations. Effects of different physical and chemical factors on the in vitro growth of these explants were studied. As far as, physical factors were concerned, it was noted that 22 2 C for in vitro growth of Solanum nigrum was used. It was also observed that the micropropagation of Solanum nigrum in liquid medium gave only 5% in vitro growth. Whereas, solidified medium (using Difco-Bacto agar as Solidifying agent) gave 80% in vitro growth for the plant. The effect of different photoperiods was also observed with 16hrs photoperiod (3000 lux) in in vitro micropropagation of Solanum nigrum. Suitable maximum pH value for micropropagation was found to be 5.7 for in vitro growth was noted for Solanum nigrum.

The present study deals with the callogenesis and micropropagation of grapes (Vitis vinifera L.) and biochemical investigation of protein content and peroxidase activity during different stages of growth. In the protocol of micropropagation, four different types of media with different concentration of auxin, cytokinin and their combinations were used. Best result was obtained with the TDZ 25.0 (uM) with 90% frequency rate. In the induction of callogenesis procedure, three different types of media concentration of auxin, cytokinin and their combination were used with leaf and node as explant. The best result observed from leaf explant obtained from 2,4-D 3.0 (mg/l) with maximum 90% frequency rate. Best result for callus formation from nodal explant obtained from 2,4-D 3.0 + BAP 1.0 + 1.0 TDZ (mg/l) with 90% frequency rate.In the estimation of soluble protein content best result of in vivo grown cultures obtained from leaf explant (0.085 0.643) in 3rd subculture. In the comparison of specific activity of peroxidase enzyme, best result of in vivo grow cultures obtained from shoot tip explant (0.00063 0.692) in 3rd subculture.