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This volume provides a complete and timely guide to the use of
adeno-associated virus (AAV) vectors for genetic manipulation of
mammalian tissues. Beginning with methods for the design and
characterization of AAV vectors, the book continues with protocols
for AAV delivery to various components of the central nervous
system, to a number of sensory systems, and to a broad range of
other tissues. Novel techniques such as ultrasound-targeted
delivery to the brain, subpial delivery to the spinal cord, and
subILM delivery to the retina are accompanied by chapters that
provide an overview and comparison of current methods for AAV
delivery to tissues such as brain, heart, liver, and lung. Written
for the highly successful Methods in Molecular Biology series,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, readily reproducible
step-by-step laboratory protocols, and tips for troubleshooting and
avoiding known pitfalls. Authoritative and comprehensive,
Adeno-Associated Virus Vectors: Design and Delivery aims to enhance
the utility of AAV vectors for targeted gene transfer to living
animals and continue the ongoing development of novel AAV-based
gene therapies for human disease.
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