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Rigor and Reproducibility in Genetics and Genomics: Peer-reviewed,
Published, Cited provides a full methodological and statistical
overview for researchers, clinicians, students, and post-doctoral
fellows conducting genetic and genomic research. Here, active
geneticists, clinicians, and bioinformaticists offer practical
solutions for a variety of challenges associated with several
modern approaches in genetics and genomics, including genotyping,
gene expression analysis, epigenetic analysis, GWAS, EWAS, genomic
sequencing, and gene editing. Emphasis is placed on rigor and
reproducibility throughout, with each section containing laboratory
case-studies and classroom activities covering step-by-step
protocols, best practices, and common pitfalls. Specific genetic
and genomic technologies discussed include microarray analysis,
DNA-seq, RNA-seq, Chip-Seq, methyl-seq, CRISPR gene editing, and
CRISPR-based genetic analysis. Training exercises, supporting data,
and in-depth discussions of rigor, reproducibility, and ethics in
research together deliver a solid foundation in research standards
for the next generation of genetic and genomic scientists.
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