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Creating bacteria with modified genetic properties allows the
specific investigation of these microorganisms.
Electrotransformation is a highly efficient and easy to apply
technique to introduce genetic material into bacterial cells. A
strong electric field is used for this purpose.
In the present manual, protocols for the transformation of about 40
strains of bacteria are described. Emphasis is placed on the
individual critical procedural steps, since the practical details
mainly depend on the bacterial strain under investigation. This
presentation together with the theoretical introductionary
chapters, allows the user to modify and adapt each protocol to
his/her own experiments. Bacterial strains with relevance in the
food industry, biotechnology, medical and veterinary fields,
agroindustry and environmental sciences are covered.
Creating bacteria with modified genetic properties allows the
specific investigation of these microorganisms.
Electrotransformation is a highly efficient and easy to apply
technique to introduce genetic material into bacterial cells. A
strong electric field is used for this purpose.
In the present manual, protocols for the transformation of about 40
strains of bacteria are described. Emphasis is placed on the
individual critical procedural steps, since the practical details
mainly depend on the bacterial strain under investigation. This
presentation together with the theoretical introductionary
chapters, allows the user to modify and adapt each protocol to
his/her own experiments. Bacterial strains with relevance in the
food industry, biotechnology, medical and veterinary fields,
agroindustry and environmental sciences are covered.
|
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