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Vinca rosea L. Syn. Catharanthus roseus L. G. Don. is an important
medicinal plant which belongs to the family Apocynaceae. The
present study deals with Micropropagation, Callogenesis, Cell
suspension culture & Antimicrobial activity of Vinca rosea L..
Explants were cultured on MS basal medium and supplemented with
different concentrations of BAP (mg/l) and NAA (mg/l) for
micropropagation whereas 2, 4-D (mg/l) and Kin (mg/l) for
callogenesis. Multiple shoots were obtained on all the
concentrations of BAP and NAA, but BAP (1 mg/l) showed best
response (90% & 80%).Similarly best callus response was
observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in
all the explants (95% from leaf, 80% from node & 60% from
fruit). Cell suspension culture have also been established in the
present study according to which the calluses gave suspensions
consisting of both the embryogenic and non-embryogenic cluster of
the cells in 2, 4- D + Kin (1+1 mg/l) and gave best response
(70%).The present study also describes the comparison of in vivo
and in vitro antimicrobial activity of Vinca rosea. Three different
pathogenic bacterial strains .) and three different fungal strains
were used.
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