Dengue is the most prevalent mosquito-borne viral infection worldwide. Over half of the world's population live in areas at risk of dengue infection. The disease is transmitted principally by Aedes spp. mosquitoes. Dengue Outbreaks were recorded in all countries of the Indian Subcontinent with Pakistan, India, Bangladesh and Sri Lanka resulting in huge number of cases and deaths. It is a disease caused by four serotypes of a same virus i.e. DENV1, DENV2, DENV3 and DENV4. In all major outbreaks, DENV2 and DENV3 were recorded as dominating and disastrous serotypes in the region. Climate is a vital factor influencing DF outbreaks; temperature, humidity, rainfall and flood play a fundamental role in DF outbreaks, as providing optimum conditions for vector population growth.Presently, prime prevention of dengue is possible only with vector control and personal protection from the bites of infected mosquitoes.

Enzymes are biological catalysts which accelerate the chemical reactions in living things without being consumed. A catalyst is a material which builds a chemical reaction goes faster, without itself being altered. A catalyst can be utilized again and again in a chemical reaction: it is available again for same function. Enzymes are exceptionally the same apart from that they are simply denatured by heating. This book discussed about the history of enzymes, Production criteria, biotechnology, classification, Kinetics and Enzyme applications. Enzyme biotechnology involves the utilization of microorganisms such as yeast, mold or bacteria, and enzymes to make goods and services is a promising field in modern biotechnology. I am looking forward to work in a professional and competitive environment where It can put my best efforts, hard work and skills to bring up the organization and myself too. I am also a life long learner and am determined to become a knowledge professional for keeping abreast with changing technology.