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Cell surface small molecules and macromolecules, such as members of
cholesterol family (including steroid hormones), the glycolipid
family (sphingolipids), the glycoprotein family (both N-linked and
O-linked), and a vast array of other receptors have been shown to
be involved in normal and abnormal cellular processes. The 11th
International Symposium on Cell Surface Macromolecules, held in
Mohali, India, in February 2017 provided a comprehensive update on
the major advances in this area. Presenting selected contributions
from this meeting, this book comprises 24 chapters, which provide
in-depth analyses of data on the role of cell surface
macromolecules in cellular function and their alterations
associated with pathological conditions. It includes comprehensive
research papers and critical overviews of the functional role of
cell surface molecules, discussing topics such as biochemical,
biophysical, and cell biological approaches to study cell membrane
molecules, and metabolism of glycoconjugates.
In vitro utilization of liposomes is now recognized as a powerful
tool in many bioscience investigations and their associated
clinical studies, e.g., liposomes in drug targeting; liposomes in
gene transport across plasma and nuclear membranes; liposomes in
enzyme therapy in patients with genetic disorders. However, before
these areas can be effectively explored, many basic areas in
liposome research require elucidation, including: (a) attachment of
liposomes to cell surfaces; (b) permeation of liposomes through the
plasma membranes; and (c) stability of liposomes in cell or nuclear
matrices. None of these areas have been exhaustively explored and
liposome researchers have ample opportunities to contribute to our
knowledge. The aim of Liposome Methods and Protocols is to bring
together a wide range of detailed laboratory protocols covering
different aspects of liposome biology in order to assist
researchers in those rapidly advancing medical fields mentioned
earlier. With this goal in mind, in each protocol chapter we have
detailed the materials to be used, followed by a step-by-step
protocol. The Notes section of each protocol is also certain to
prove particularly useful, since the authors include
troubleshooting tips straight from their benchtops, valuable
information that is seldom given in restricted methods sections of
standard research journals. For this reason we feel that the book
will prove especially useful for all researchers in the liposome
field.
In vitro utilization of liposomes is now recognized as a powerful
tool in many bioscience investigations and their associated
clinical studies, e.g., liposomes in drug targeting; liposomes in
gene transport across plasma and nuclear membranes; liposomes in
enzyme therapy in patients with genetic disorders. However, before
these areas can be effectively explored, many basic areas in
liposome research require elucidation, including: (a) attachment of
liposomes to cell surfaces; (b) permeation of liposomes through the
plasma membranes; and (c) stability of liposomes in cell or nuclear
matrices. None of these areas have been exhaustively explored and
liposome researchers have ample opportunities to contribute to our
knowledge. The aim of Liposome Methods and Protocols is to bring
together a wide range of detailed laboratory protocols covering
different aspects of liposome biology in order to assist
researchers in those rapidly advancing medical fields mentioned
earlier. With this goal in mind, in each protocol chapter we have
detailed the materials to be used, followed by a step-by-step
protocol. The Notes section of each protocol is also certain to
prove particularly useful, since the authors include
troubleshooting tips straight from their benchtops, valuable
information that is seldom given in restricted methods sections of
standard research journals. For this reason we feel that the book
will prove especially useful for all researchers in the liposome
field.
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