This study was carried out at the Department of Microbiology and Biotechnology Karnatak University, Dharwad. The bacterial strains were collected from various clinical samples like urine, infected blood, pus, wound swabs, catheters and cerebrospinal fluid (CSF) from different inpatients of Karnatak Institute of Medical sciences (KIMS), Civil Hospital, and local hospitals and Nursing Homes of Hubli-Dharwad. One thousand fifty individuals were included in the study, seven hundred and fifty males, and three hundred females.For isolation and identification of Staphylococcus aureus the samples were inoculated on nutrient agar and selective medium, mannitol salt agar (MSA). The characteristic colonies producing golden yellow or cream, round, raised regular colonies on nutrient agar and yellow coloration around MSA were presumptively selected as total of 675 Staphylococcus spp. isolates were procured. These isolates were subjected for cultural, microscopical and biochemical analysis for confirmation

Viral hepatitis is an inflammation of the liver due to viral infection and there are groups of virus that affects the liver of which hepatitis B and C viruses are the causative agents of sever form of liver disease with high rate of mortality. Medical waste handlers who undergo collection, transportation, and disposal of medical wastes in the health institutions are at risk of exposure to acquire those infections which transmit mainly as a result of contaminated blood and other body fluids including injury with sharp instruments, splash to the eye or mucous membrane. A cross-sectional study was conducted in government health institutions in Gondar town. Prevalence rates of HBV and HCV were significantly higher in medical waste handlers than those in non-medical waste handlers examined. Regularly supply of personal protection equipments, training, pre and post-exposure protection of medical waste handlers, may significantly reduce the risk of acquiring infectious agents.