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The book presents the latest advances and research findings in the fields of computational science and communication. The areas covered include smart innovation; systems and technologies; embedded knowledge and intelligence; innovation and sustainability; advanced computing; and networking and informatics. It also focuses on the knowledge-transfer methodologies and the innovation strategies employed to make these effective. This fascinating compilation appeals to researchers, academics and engineers around the globe.
This book presents the latest advances and research findings in the fields of computational science and communication presented at the International Conference on Smart Innovations in Communications and Computational Sciences (ICSICCS 2020). The areas covered include smart innovation; systems and technologies; embedded knowledge and intelligence; innovation and sustainability; advanced computing; networking and informatics. It also focuses on the knowledge-transfer methodologies and the innovation strategies employed to make these effective. This fascinating compilation appeals to researchers, academics and engineers around the globe.
The objective of this study was to understand the pitfalls of the poor oocyte cleavage and subsequent embryo production by assessing the developmental competence of the oocytes in buffalo using parthenogenesis as a model which was compared with IVF (natural activation). Buffalo ovaries were collected from slaughter house and matured oocytes were subjected to either IVF (control) or chemical activation (treatment) 7% ethanol (ET)for 7 min ] 2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10 ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically activated oocytes were cultured in mSOF medium for 8 days to study embryo development.In conclusion, the results of parthenogenesis, revealed that buffalo oocytes had better inherent developmental competence and the poor cleavage and embryo development following IVF, in our study, may partly be due to the poor quality of frozen-thawed sperm, improper sperm capacitation and/ or fertilization. Further, high ambient temperature adversely affected the cleavage and embryo development following IVF.
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