This textbook for graduate students presents fundamental and essential principles of forensic biology. It covers the theory, principles, and applications of forensic biology, focusing on the easier understanding of the applicability of the topics. It discusses the subject with an aim to enhance the theoretical and practical knowledge of the subject and explore the potentials of the fields in modern-day crime scene investigation for researchers and practitioners of the field. The book is supplemented with real-life case studies from national and international cases, significant to the discipline or unique approach to evidence analysis. Notably, the textbook discusses forensic sample analysis, emerging trends and new technologies, and legal and ethical concepts about forensic investigations. It further presents the history and development of forensic DNA profiling and the role of DNA databases in forensic investigations. It elucidates the applications of nanotechnology in forensics and examines the role of forensics in attributing acts of bioterrorism or bioproliferation.