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This book focuses on the state-of-the-art of biosensor research and development for specialists and non-specialists. It introduces the fundamentals of the subject with relevant characteristics of transducer elements, as well as biochemical recognition molecules. This book is ideal for researchers of nanotechnology, materials science and biophysics.
There is a worldwide effort towards the development of
bioanalytical devices that can be used for detection,
quantification and monitoring of specific chemical species. In this
context, biosensors represent an emerging trend in the diagnostics
industry. A biosensor is a device that has a biological sensing
element either intimately connected to or integrated within a
transducer. The aim is to produce a digital electronic signal that
is proportional to the concentration of a specific chemical or a
set of chemicals. Biosensors are specific, rapid, cost-effective
and easy to use devices that can be employed with minimal sample
treatment. Biosensors have applications in many areas such as
biotechnology, healthcare, pollution monitoring, food and
agriculture product monitoring, the pharmaceuticals industry and
defense.
Sol-gel derived metal oxide nanostructures based biosensors have shown potential application in various fields including mainly environmental and biological screening/monitoring. Composite film of CeO2-SiO2 has been fabricated using sol-gel technique to prepare enzymatic and DNA biosensor for the detection of triglyceride and cypermethrin, respectively. Lipase (Lip) and double stranded calfthymus (dsCT) was immobilized via physiosorption for the biosensing of respected analyte. The electrical response studies of Lip/CeO2-SiO2/ITO carried out using CV reveals linearity from 50 to 400 mg/dL with high sensitivity (2.28 mA/mg/dL). Electrochemical response studies of dsCT-DNA/CeO2-SiO2/ITO bioelectrode carried out as CM concentration using DPV technique exhibit detection limit up to 0.0025 ppm with response time of 30 sec. The finding of this study suggests that sol-gel derived metal oxides nanostructures of desired optical and electrical properties can be used for biosensor development to detect biomarkers in physiological range.
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