This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Set against the backdrop of a rapidly fissuring disciplinary landscape where poetry and science are increasingly viewed as irreconcilable and unrelated, Bernhard Kuhn's study uncovers a previously ignored, fundamental connection between autobiography and the natural sciences. Examining the autobiographies and scientific writings of Rousseau, Goethe, and Thoreau as representative of their ages, Kuhn challenges the now entrenched thesis of the "two cultures." Rather, these three writers are exemplary in that their autobiographical and scientific writings may be read not as separate or even antithetical but as mutually constitutive projects that challenge the newly emerging boundaries between scientific and humanistic thought during the Romantic period. Reading each writer's life stories and nature works side by side-as they were written-Kuhn reveals the scientific character of autobiographical writing while demonstrating the autobiographical nature of natural science. He considers all three writers in the context of scientific developments in their own times as well as ours, showing how each one marks a distinctive stage in the growing estrangement of the arts and sciences, from the self-assured epistemic unity of Rousseau's time, to the splintering of disciplines into competing ways of knowing under the pressures of specialization and professionalization during the late Romantic age of Thoreau. His book thus traces an unfolding drama, in which these writers and their contemporaries, each situated in an intellectual landscape more fragmented than the last, seek to keep together what modern culture is determined to break apart.

Set against the backdrop of a rapidly fissuring disciplinary landscape where poetry and science are increasingly viewed as irreconcilable and unrelated, Bernhard Kuhn's study uncovers a previously ignored, fundamental connection between autobiography and the natural sciences. Examining the autobiographies and scientific writings of Rousseau, Goethe, and Thoreau as representative of their ages, Kuhn challenges the now entrenched thesis of the "two cultures." Rather, these three writers are exemplary in that their autobiographical and scientific writings may be read not as separate or even antithetical but as mutually constitutive projects that challenge the newly emerging boundaries between scientific and humanistic thought during the Romantic period. Reading each writer's life stories and nature works side by side-as they were written-Kuhn reveals the scientific character of autobiographical writing while demonstrating the autobiographical nature of natural science. He considers all three writers in the context of scientific developments in their own times as well as ours, showing how each one marks a distinctive stage in the growing estrangement of the arts and sciences, from the self-assured epistemic unity of Rousseau's time, to the splintering of disciplines into competing ways of knowing under the pressures of specialization and professionalization during the late Romantic age of Thoreau. His book thus traces an unfolding drama, in which these writers and their contemporaries, each situated in an intellectual landscape more fragmented than the last, seek to keep together what modern culture is determined to break apart.

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.