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This book was originated from a series of lectures given in a
course on the physical properties of biological membranes and their
functional implica tions. The course was intended to allow students
to get acquainted with the physical techniques used to study
biological membranes. The experience was valuable and we feel that
a detailed description of the procedures used and of various
examples of the results obtained allowed many students to become
familiar with a theme that is not often part of regular courses on
membrane physiology or biophysics. This book is designed as a
tutorial guide for graduate students interested in understanding
how physical methods can be utilized to study the proper ties of
biological membranes. It includes first a detailed description of
applications of physical techniques-such as X-ray fiber diffraction
methods (Chapter 1), 2H and 13C NMR spectroscopy (Chapter 2), and
calorimetry (Chapter 3)-in the study of the properties of lipid
model membranes. A description of how to measure molecular mobility
in membranes (Chapter 4) follows, and the book concludes with three
chapters in which biological membranes are the subject of study.
Chapter 5 deals with the acetylcholine receptor and its membrane
environment; Chapter 6 discusses how fluorescence techniques can be
applied in the study of the calcium ATPase of sarcoplasmic
reticulum; and Chapter 7 explains how protein lipid interactions
modulate the function of the sodium and proton pumps."
The present volume originated from the workshop "Transduction in
Biological Sys tems," held at the Marine Biological Station of the
Universidad de Valparaiso, Mon temar, Chile, May 23-30, 1988, and
contains contributions from most of the partici pants in the
workshop. The title of both the workshop and the book reflects
accurately the central theme discussed during several days of
intense debate and profound intellectual exchange in the peaceful
environment offered by the central coast of Chile. It was apparent
that the workshop was a great success-a sentiment expressed by many
seasoned attendees, some of whom dared opinions as strong as "It
was the best ever." There is no single reason to explain why this
workshop was so successful. Certainly instrumental was the
incredible effort displayed by the Chilean Organizing Committee in
selecting adequate facilities and in organizing social events that
supplemented the scien tific sessions and provided an authentic
fraternal environment for the participants. Equally important were
the foreign participants, who enthusiastically gave of their time
to take part in the event, and the students, who came from Chile as
well as from several other Latin American countries, and who
applied the necessary pressure in their repeated demands for
scientific clarity, accuracy, and sincerity."
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