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This volume provides readers with methods and protocols for
understanding the development of recombinant viruses and their use
as vaccines platforms. Recombinant Virus Vaccines: Methods and
Protocols details the use of recombinant vaccines that are employed
to either produce immunogens in vitro or elicit antibody production
in vivo. The chapters in this book are divided into four parts:
Part I explores double-stranded DNA viruses; Part II discusses
negative sense single-stranded RNA viruses; Part III talks about
positive sense single-stranded RNA viruses; and Part IV describes
bacteriophages. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Thorough
and cutting-edge, Recombinant Virus Vaccines: Methods and Protocols
is a valuable resource for scientists and clinicians who are
interested in learning about and adopting methods for use in basic
and biomedical research directed toward generating and developing
recombinant viral vaccines.
This volume has been assembled for scientists interested in basic
and applied biomedical research directed toward understanding the
development, genetics and function of cardiomyocytes. The methods
and protocols contained within it address cell culture techniques,
cardiomyocyte differentiation and redifferentiation, experimental
induction of cardiomyopathies, introducing genes into
cardiomyocytes, genomic approaches to the understanding
cardiomyocytes, cryopreservation of neonatal cardiomyocytes and
modeling of cardiomyocyte function. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls. Practical and current, Cardiomyocytes:
Methods and Protocols explores complimentary areas of cardiomyocyte
science that, taken together, can inform individuals with a broad
range of interests.
This volume provides readers with methods and protocols for
understanding the development of recombinant viruses and their use
as vaccines platforms. Recombinant Virus Vaccines: Methods and
Protocols details the use of recombinant vaccines that are employed
to either produce immunogens in vitro or elicit antibody production
in vivo. The chapters in this book are divided into four parts:
Part I explores double-stranded DNA viruses; Part II discusses
negative sense single-stranded RNA viruses; Part III talks about
positive sense single-stranded RNA viruses; and Part IV describes
bacteriophages. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Thorough
and cutting-edge, Recombinant Virus Vaccines: Methods and Protocols
is a valuable resource for scientists and clinicians who are
interested in learning about and adopting methods for use in basic
and biomedical research directed toward generating and developing
recombinant viral vaccines.
This volume has been assembled for scientists interested in basic
and applied biomedical research directed toward understanding the
development, genetics and function of cardiomyocytes. The methods
and protocols contained within it address cell culture techniques,
cardiomyocyte differentiation and redifferentiation, experimental
induction of cardiomyopathies, introducing genes into
cardiomyocytes, genomic approaches to the understanding
cardiomyocytes, cryopreservation of neonatal cardiomyocytes and
modeling of cardiomyocyte function. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls. Practical and current, Cardiomyocytes:
Methods and Protocols explores complimentary areas of cardiomyocyte
science that, taken together, can inform individuals with a broad
range of interests.
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