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This volume brings together the most widely used and important
protocols currently being employed in researching and understanding
bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall
Homeostasis cover a variety of subjects, such as: modern microscopy
techniques and other biophysical methods used to characterize the
subcellular structure of the bacterial cell wall; high-throughput
approaches that can be used to identify all the genes and proteins
that participate in the correct functioning of an organism's cell
wall; protocols for assaying individual gene products for specific
cell wall functions or identify chemicals with inhibitory activity
against the cell wall; and methods for analyzing the non-protein
components of the cell wall and the increasing use of computational
approaches for predicting and modeling cell wall related functions
and processes. Written in the highly successful Methods in
Molecular Biology series format, chapters include introduction to
their respective topics, lists of the necessary material and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Thorough
and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and
Protocols emphasizes the diversity of the research taking place in
bacterial cell wall homeostasis, and explains how the integration
of information from across multiple disciplines is going to be
essential if a holistic understanding of this important process is
to be obtained.
This volume brings together the most widely used and important
protocols currently being employed in researching and understanding
bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall
Homeostasis cover a variety of subjects, such as: modern microscopy
techniques and other biophysical methods used to characterize the
subcellular structure of the bacterial cell wall; high-throughput
approaches that can be used to identify all the genes and proteins
that participate in the correct functioning of an organism's cell
wall; protocols for assaying individual gene products for specific
cell wall functions or identify chemicals with inhibitory activity
against the cell wall; and methods for analyzing the non-protein
components of the cell wall and the increasing use of computational
approaches for predicting and modeling cell wall related functions
and processes. Written in the highly successful Methods in
Molecular Biology series format, chapters include introduction to
their respective topics, lists of the necessary material and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Thorough
and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and
Protocols emphasizes the diversity of the research taking place in
bacterial cell wall homeostasis, and explains how the integration
of information from across multiple disciplines is going to be
essential if a holistic understanding of this important process is
to be obtained.
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