New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body.

This book presents a study into the art of Jiangnan classical garden. Jiangnan ("the south of the Yangtze River") refers to the water network region along the lower reaches of the Changjiang River (formerly known as Yangtze River), where Jiangsu Province Chinese gardens were primarily constructed during the 16th and 17th centuries of the later Ming and early Qing dynasties. The Jiangnan garden, an architectural space where artificial and natural elements are combined, represents the elite of classical Chinese gardens and serves as a prime exemplar for its northern counterpart, the Ming and Qing imperial gardens. The book pursues an interdisciplinary approach, combining historical information with case studies and other methods. Charts and pictures are used to supplement and reinforce the conclusions drawn from the macro narrative, enhancing the authenticity and readability of the historical monographs. It represents the first study of the classical art of landscape design in China, offering readers an insightful introduction.

Capillary electrophoresis (CE) and microfluidic chip (MC) devices are relatively mature technologies, but this book demonstrates how they can be integrated into a single, revolutionary device that can provide on-site analysis of samples when laboratory services are unavailable. By introducing the combination of CE and MC technology, Microfluidic Chip-Capillary Electrophoresis Devices broadens the scope of chemical analysis, particularly in the biomedical, food, and environmental sciences. The book gives an overview of the development of MC and CE technology as well as technology that now allows for the fabrication of MC-CE devices. It describes the operating principles that make integration possible and illustrates some achievements already made by the application of MC-CE devices in hospitals, clinics, food safety, and environmental research. The authors envision further applications for private and public use once the proof-of-concept stage has been passed and obstacles to increased commercialization are addressed. A summary of the current methodology and application of MC-CE devices, including their merits, limitations, and potential, closes the book. It highlights the path to be taken for future development of MC-CE and anticipates new advances in technology that will increase their performance and use as powerful analytical tools. With enhanced power, their scope of applications will widen and yield new benefits to researchers and the general public.

This book presents a study into the art of Jiangnan classical garden. Jiangnan (“the south of the Yangtze River�) refers to the water network region along the lower reaches of the Changjiang River (formerly known as Yangtze River), where Jiangsu Province Chinese gardens were primarily constructed during the 16th and 17th centuries of the later Ming and early Qing dynasties. The Jiangnan garden, an architectural space where artificial and natural elements are combined, represents the elite of classical Chinese gardens and serves as a prime exemplar for its northern counterpart, the Ming and Qing imperial gardens. The book pursues an interdisciplinary approach, combining historical information with case studies and other methods. Charts and pictures are used to supplement and reinforce the conclusions drawn from the macro narrative, enhancing the authenticity and readability of the historical monographs. It represents the first study of the classical art of landscape design in China, offering readers an insightful introduction.

This volume of Advances in Intelligent Systems and Computing contains accepted papers presented at ICGEC 2014, the 8th International Conference on Genetic and Evolutionary Computing. The conference this year was technically co-sponsored by Nanchang Institute of Technology in China, Kaohsiung University of Applied Science in Taiwan, and VSB-Technical University of Ostrava. ICGEC 2014 is held from 18-20 October 2014 in Nanchang, China. Nanchang is one of is the capital of Jiangxi Province in southeastern China, located in the north-central portion of the province. As it is bounded on the west by the Jiuling Mountains, and on the east by Poyang Lake, it is famous for its scenery, rich history and cultural sites. Because of its central location relative to the Yangtze and Pearl River Delta regions, it is a major railroad hub in Southern China. The conference is intended as an international forum for the researchers and professionals in all areas of genetic and evolutionary computing.

New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body.

Capillary electrophoresis (CE) and microfluidic chip (MC) devices are relatively mature technologies, but this book demonstrates how they can be integrated into a single, revolutionary device that can provide on-site analysis of samples when laboratory services are unavailable. By introducing the combination of CE and MC technology, Microfluidic Chip-Capillary Electrophoresis Devices broadens the scope of chemical analysis, particularly in the biomedical, food, and environmental sciences. The book gives an overview of the development of MC and CE technology as well as technology that now allows for the fabrication of MC-CE devices. It describes the operating principles that make integration possible and illustrates some achievements already made by the application of MC-CE devices in hospitals, clinics, food safety, and environmental research. The authors envision further applications for private and public use once the proof-of-concept stage has been passed and obstacles to increased commercialization are addressed. A summary of the current methodology and application of MC-CE devices, including their merits, limitations, and potential, closes the book. It highlights the path to be taken for future development of MC-CE and anticipates new advances in technology that will increase their performance and use as powerful analytical tools. With enhanced power, their scope of applications will widen and yield new benefits to researchers and the general public.

Serum Pharmacochemistry of Traditional Chinese Medicine: Technologies, Strategies and Applications provides a valuable and indispensable guide on the latest methods, research advances, and applications in this area. Chapters offer cutting-edge information on pharmacokinetics and pharmacodynamics, analytical chemistry, traditional medicine, natural products, bioinformatics, new technologies, therapeutic applications, and more. For researchers and students in academia and industry, this book provides a hands-on description of experimental techniques, along with beneficial guidelines to help advance research in the fields of Traditional Chinese Medicine and drug development.