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This detailed volume compiles state-of-the-art protocols that will
serve as recipes for scientists researching collagen, an abundant
protein with great importance to health and disease, as well as in
applications like food, cosmetics, pharmaceuticals, cosmetic
surgery, artificial skin, and glue. Beginning with a section on in
vitro models for the characterization of collagen formation, the
book continues by highlighting large-scale analysis of collagen
with mass spectrometry in order to elucidate the proteomics,
degradomics, interactomes, and cross-linking of collagen, high
resolution imaging approaches for collagen by the use of scanning
electron microscopy and multiphoton imaging, as well as the role of
collagen during physiological and pathological conditions. Written
for the highly successful Methods in Molecular Biology series,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Collagen:
Methods and Protocols is an ideal guide to high quality and
repeatable protocols in this vital field of study.
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