Diversity within and among living organisms is both a biological impera tive and a biological conundrum. Phenotypic and genotypic diversity is the critical currency ofecological interactions and the evolution of life. Thus, it is not unexpected to find vast phytochemical diversity among plants. However, among the most compelling questions which arise among those interested in ecological phytochemistry is the extent, nature, and reasons for the diversity of chemieals in plants. The idea that natural products (secondary metabolites) are accidents of metabolism and have no biological function is an old one which has resurfaced recently under a new term "redundancy. " Redundancy in the broader sense can be viewed as duplication of effort. The co-occurrence of several classes of phytochemieals in a given plant may be redundancy. Is there unnecessary duplication of chemical defense systems and ifso, why? What selective forces have produced this result? On the other hand, why does the same compound often have multiple functions? At a symposium of the Phytochemical Society of North America held in August 1995, in Sault Ste. Marie, Ontario, Canada, the topic "Phytochernical Redundancy in Ecological Interactions" was discussed. The chapters in this volume are based on that symposium. They both stimulate thought and provide some working hypotheses for future research. It is being increasingly recognized that functional diversity and multiplicity of function of natural products is the norm rather than the exception."

Diversity within and among living organisms is both a biological impera tive and a biological conundrum. Phenotypic and genotypic diversity is the critical currency ofecological interactions and the evolution of life. Thus, it is not unexpected to find vast phytochemical diversity among plants. However, among the most compelling questions which arise among those interested in ecological phytochemistry is the extent, nature, and reasons for the diversity of chemieals in plants. The idea that natural products (secondary metabolites) are accidents of metabolism and have no biological function is an old one which has resurfaced recently under a new term "redundancy. " Redundancy in the broader sense can be viewed as duplication of effort. The co-occurrence of several classes of phytochemieals in a given plant may be redundancy. Is there unnecessary duplication of chemical defense systems and ifso, why? What selective forces have produced this result? On the other hand, why does the same compound often have multiple functions? At a symposium of the Phytochemical Society of North America held in August 1995, in Sault Ste. Marie, Ontario, Canada, the topic "Phytochernical Redundancy in Ecological Interactions" was discussed. The chapters in this volume are based on that symposium. They both stimulate thought and provide some working hypotheses for future research. It is being increasingly recognized that functional diversity and multiplicity of function of natural products is the norm rather than the exception.

The influence of compounds in the environment on the chemistry of plants is a topic which has economic and scientific implications of global importance. Selected presentations in this symposium covered several topics within this immense field, inclusive of air, soil, and aquatic sources of the compounds. As demonstrated in Chapter 4 by O'Keeffe et al. we have not restricted the discussion solely to negative aspects of anthropogenic compounds. Nor could we begin to cover comprehensively all major classes of environmental compounds in the air, soil or water that may have an effect on the phytochemistry of plants. Our intent was to focus on some of the timely and well publicized environmental constituents such as ozone, sulfur dioxide, acid rain, and others, to provide an authoritative publication specifically related to environ mental modifications of plant chemistry. The concept of this symposium originated with the Executive Committee of the Phytochemical Society of North America in 1983. It was brought to fruition during July 13-17, 1986 on the campus of the University of Maryland at the annual meeting of the PSNA through the efforts of the Symposium Committee composed of James A. Saunders and Lynn Kosak-Channing. Financial support for this meeting was provided by the Phytochemical Society of North America, as well as by generous contributions from E. I. du Pont de Nemours & Company and the U. S. Department of Agriculture. The Organizing Committee, consisting of J. A. Saunders (Chair), J. M. Gillespie, L. Kosak-Channing, E. H. Lee, J. P."

Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.
Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques.
Key Features
* Covers fundamentals of electroporation and electrofusion in detail
* Molecular events
* Mechanisms
* Kinetics
* Gives extensive practical information
* The latest applications
* Controlling parameters to maximize efficiency
* Available instrumentation
* Presents applications of electroporation and electrofusion in current research situations
* State-of-the-art modifications to electrical pulses and generators
* Application of electroporation and electrofusion to unique, alternative cell and tissue types
* Gives straightforward, detailed, easy-to-follow protocols for
* Formation of human hybridomas
* Introduction of genetic material into plant cells and pollen
* Transfection of mammalian cells
* Transformation of bacteria, plants, and yeast
* Production of altered embryos
* Optimization of electroporation by using reporter genes
* Comprehensive and up-to-date
* Convenient bench-top format
* Approximately 125 illustrations complement the text
* Complete references with article titles
* Written by leading authorities in electroporation and electrofusion