This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field.

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field.