In numerous fields of science, work, and everyday life, humans and machines have been increasingly entangled, developing an ever-growing toolbox of interactions. These entanglements affect our daily lives and pose possibilities as well as restrictions, chances as well as challenges. The contributions of this volume tackle related issues by adopting a highly interdisciplinary perspective. How do digitalization and artificial intelligence affect gender relations? How can intersectionality be newly understood in an increasingly internationally networked world? This volume is a collection of contributions deriving from the "Interdisciplinary Conference on the Relations of Humans, Machines and Gender" which took place in Braunschweig (October 16-19, 2019). It also includes the keynotes given by Cecile Crutzen, Galit Wellner and Helen Verran.