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There have been many important advances in our understanding of
biological membrane structure and function over the last decade.
Much of this progress has been driven by the development of new
techniques for studying membrane components and their interactions.
Tradition ally, the investigation of membranes has largely occurred
within the domains of biochemistry, physical chemistry, and cell
biology; but many of the most significant advances have resulted
from an expansion into other disciplines, such as molecular
biology, immunology, and the clinical sciences. In these two
volumes we have attempted to bring together some of these
techniques-to combine the old and the new. Biomembrane Protocols:
II. Architecture and Function is concerned exclusively with the
architecture and activities of membranes, whereas the companion
volume, Biomembrane Protocols: I. Isolation and Analysis, deals
with their isolation and compositional analysis. The aim of each
chapter is to provide detailed technical and meth odological
information that will allow the reader to perform the tech nique
successfully, without the need to consult other texts. Detailed
reviews of membrane structure and function are not included, except
where they are relevant to the choice or efficacy of a particular
proce dure. In addition to Materials and Methods sections, each
chapter has a Notes section that explains the reasons for taking
certain steps and pro vides practical tips for applying the
techniques to other systems.
Expert practitioners combine the traditional basic techniques of
membrane isolation and analysis with modern advances in molecular
biology, immunology, and clinical sciences to devise analytical
techniques that are successful for expert and novice alike. The
techniques are used in the isolation and compositional analysis of
membranes and address animal, plant, and microbial cells. The
topics covered in this first volume include the isolation of
subcellular fractions, centrifugation, the extraction and
quantitation of lipids, the separation and analysis of proteins,
and the isolation and characterization of glycosylated molecules,
glycoproteins, proteoglycans, and glycolipids.
There have been many important advances in our understanding of
biological membrane structure and function over the last decade.
Much of this progress has been driven by the development of new
techniques for studying membrane components and their interactions.
Tradition- ally, the investigation of membranes has largely
occurred within the domains of biochemistry, physical chemistry,
and cell biology; but many of the most significant advances have
resulted from an expansion into other disciplines, such as
molecular biology, immunology, and the clinical sciences. In these
two volumes we have attempted to bring together some of these
techniques-to combine the old and the new. Biomembrane Protocols:
II. Architecture and Function is concerned exclusively with the
architecture and activities of membranes, whereas the companion
volume, Biomembrane Protocols: I. Isolation and Analysis, deals
with their isolation and compositional analysis. The aim of each
chapter is to provide detailed technical and meth- odological
information that will allow the reader to perform the tech- nique
successfully, without the need to consult other texts. Detailed
reviews of membrane structure and function are not included, except
where they are relevant to the choice or efficacy of a particular
proce- dure. In addition to Materials and Methods sections, each
chapter has a Notes section that explains the reasons for taking
certain steps and pro- vides practical tips for applying the
techniques to other systems.
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