This book describes modern preparative chromatography techniques and their application in the separation of natural products, synthetic intermediates, metabolites, macromolecules, and biologically active substances, e.g. new lead compounds from microorganisms and both marine and terrestrial higher organisms. The authors focus their book on the applications and aim to provide the reader with a rapid idea on how to obtain milligram or gram quantities for pure constituents from complex mixtures with minimum effort. Descriptions of apparatus and operation procedures together with numerous examples of actual separations - often widely scattered in literature - are provided. This completely revised second edition has been enlarged by chapters on macromolecule and and enantiomer separation and on preparative GC.

This is the first comprehensive monograph to look in depth at saponins. Saponins are glycosides of triterpenes, steroids or steroid alkaloids which have a very wide distribution in plants and some marine organisms. Their biological activity includes haemolysis and fish poisoning, and the steroid saponins are essential for the manufacture of oral contraceptives and sex hormones. Saponins have also been exploited commercially as soap substitutes and in several other industrial applications. This book gives detailed information on the occurrence and distribution of saponins, their structural types, isolation, analysis and structure determination. Biological and pharmacological activities are discussed, as are aspects of commercial and industrial use. The volume provides a valuable source of data for the different classes of compound and includes and extensive list of references.

This is the first comprehensive monograph to look in depth at saponins. Saponins are glycosides of triterpenes, steroids or steroid alkaloids which have a very wide distribution in plants and some marine organisms. Their biological activity includes haemolysis and fish poisoning, and the steroid saponins are essential for the manufacture of oral contraceptives and sex hormones. Saponins have also been exploited commercially as soap substitutes and in several other industrial applications. This book gives detailed information on the occurrence and distribution of saponins, their structural types, isolation, analysis and structure determination. Biological and pharmacological activities are discussed, as are aspects of commercial and industrial use. The volume provides a valuable source of data for the different classes of compound and includes and extensive list of references.