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This volume provides a collection of protocols for the common
experimental approaches used in the in the burgeoning field of
c-di-GMP-dependent signaling. The chapters, divided into eight
major parts, guide readers through methods on synthesis, detection,
quantitation, modulation of the levels of c-di-GMP present in
cells, procedures to detect and evaluate the interaction of
c-di-GMP, and up and coming approaches focusing on the inhibition
of c-di-GMP signaling.Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, c-di-GMP Signaling: Methods and
Protocols aims to inspire researchers to try new approaches.
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