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Glycosaminoglycans: Chemistry and Biology emphasizes several areas
of glycosaminoglycan research especially analysis and application
of GAGs using a variety of approaches. Chapters focus on chemical
and enzymatic synthesis of GAGs for therapeutic purposes;
biophysical and biochemical methods for studying GAG-protein
interactions; molecular approaches for modulating and defining GAG
biosynthesis; informatics approaches for deciphering GAG code;
computational approaches for establishing specific and/or
non-specific interactions and genetic and biochemical tools for
manipulating/visualizing glycosaminoglycan expression and studying
their functions in a variety of model organisms. Written in the
highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and key tips on troubleshooting
and avoiding known pitfalls. Authoritative and practical,
Glycosaminoglycans: Chemistry and Biology serves as a valuable
manual for cutting edge methodologies and practical tips to
overcome any obstacles with experimentation pertaining to chemistry
and biology of glycosaminoglycans.
Glycosaminoglycans: Chemistry and Biology emphasizes several areas
of glycosaminoglycan research especially analysis and application
of GAGs using a variety of approaches. Chapters focus on chemical
and enzymatic synthesis of GAGs for therapeutic purposes;
biophysical and biochemical methods for studying GAG-protein
interactions; molecular approaches for modulating and defining GAG
biosynthesis; informatics approaches for deciphering GAG code;
computational approaches for establishing specific and/or
non-specific interactions and genetic and biochemical tools for
manipulating/visualizing glycosaminoglycan expression and studying
their functions in a variety of model organisms. Written in the
highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and key tips on troubleshooting
and avoiding known pitfalls. Authoritative and practical,
Glycosaminoglycans: Chemistry and Biology serves as a valuable
manual for cutting edge methodologies and practical tips to
overcome any obstacles with experimentation pertaining to chemistry
and biology of glycosaminoglycans.
This second edition volume expands on the previous edition with new
information on the structural analysis of glycosaminoglycans
(GAGs); chemical and enzymatic synthesis of GAGs; biophysical,
biochemical, and computational analysis of GAG-protein
interactions; molecular and genetic approaches to manipulating GAG
cell biology; and molecular and genetic methods involving animal
models and organoids. This volume also offers insight and guidance
to non-glycoscience researchers who are performing advanced
experiments on GAGs. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. \
Cutting-edge and authoritative, Glycosaminoglycans: Methods and
Protocols, Second Edition is a valuable experimental manual for
practicing researchers including graduate and post-doctoral
fellows, and provides them with the latest methodologies and
practical tips to overcoming barriers in understanding the
chemistry and biology of GAGs.
This second edition volume expands on the previous edition with new
information on the structural analysis of glycosaminoglycans
(GAGs); chemical and enzymatic synthesis of GAGs; biophysical,
biochemical, and computational analysis of GAG-protein
interactions; molecular and genetic approaches to manipulating GAG
cell biology; and molecular and genetic methods involving animal
models and organoids. This volume also offers insight and guidance
to non-glycoscience researchers who are performing advanced
experiments on GAGs. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. \
Cutting-edge and authoritative, Glycosaminoglycans: Methods and
Protocols, Second Edition is a valuable experimental manual for
practicing researchers including graduate and post-doctoral
fellows, and provides them with the latest methodologies and
practical tips to overcoming barriers in understanding the
chemistry and biology of GAGs.
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