Thirteen words, thirteen protagonists, thirteen stories. This special journey crosses different regions and tells of mastery, know-how, emotions, and characters that honor ancient Italian traditions. Gaggia, the brand that invented espresso machines for bars and homes, wanted to represent Italian creativity in 13 stories. To achieve this, it selected the same number of contemporary artisans, including a blacksmith, a motorcycle restorer, a ceramist, a hatter, a tailor, and a creator of floral sets, and depicted them in the pages of the book through the images of Stefania Giorgi and the words of Laura Maggi. In this tale, coffee is an often invisible link; it represents the irreplaceable value of reflection, of time rediscovered, of having a break.

The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, "omic" approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, "omic" approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.