Athymic Nude Rat Brain Atlas provides the first stereotaxic brain atlas of the athymic nude rat (Crl:NIH-Foxn1rnu), a T-cell deficient mouse model frequently used for experimental studies and pre-clinical safety and efficacy studies. This 2D vector-based atlas contains coronal, sagittal and horizontal brain sections of an athymic rat brain compared to SD rats both processed in parallel, stained with cresyl violet (Nissl) and placed in 3D using Cinema 4D using "flat" skull Bregma and Lambda as anatomical landmarks for correct placement in the 3D environment. Each brain structure is illustrated as a volume, making it easy to compare the two rat strains, allow for precise targeting, and better calculate coordinates to target specific structures for toxin, virus or cell delivery using stereotaxic surgery. This atlas will serve the needs of any neuroscientist who wishes to work with these mice in experimental and pre-clinical studies. In addition, it will serve as a valuable resource for the wider research community using nude rats.