This volume provides properties, biological function, methods, and approaches for manipulating nAChRs in different organisms. Nicotinic Acetylcholine Receptor Technologies guides readers through molecular techniques and behavioral tests used to investigate nicotinic drugs, chronoamperometry, emerging technologies and methods for the analysis of nAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectral confocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutional relation for most nAChR subunits, in both vertebrate and invertebrate species. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Nicotinic Acetylcholine Receptor Technologies aims to ensure successful results in the further study of this vital field.

This volume provides properties, biological function, methods, and approaches for manipulating nAChRs in different organisms. Nicotinic Acetylcholine Receptor Technologies guides readers through molecular techniques and behavioral tests used to investigate nicotinic drugs, chronoamperometry, emerging technologies and methods for the analysis of nAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectral confocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutional relation for most nAChR subunits, in both vertebrate and invertebrate species. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Nicotinic Acetylcholine Receptor Technologies aims to ensure successful results in the further study of this vital field.

The primary objective of this book is to provide readers with up-to-date knowledge of recent advances in serotonin research across different fields. The research topics include the following aspects: The first chapter provides novel evidence supporting the involvement of genetic variants in the serotonin transporter and receptor genes in the aetiology of nicotine dependence and alcoholism through gene-by-gene interaction. The second chapter provides an update on genes and genetic variants in the serotonergic network that have been implicated in sudden infant death syndrome. The third chapter discusses how aggressive social stress such as absence of electricity, cold weather, shortage of food, and social isolation influence the development of chronic stress, pathological aggression, and formation of killer and self-killer organisms and how the distribution and concentration of serotonin in the brain are changed along with these physiological and pathological conditions. Chapter Four shows that serotonin signalling influences many developmental processes both in the central nervous system and in the peripheral organs, and Chapter Five reviews new evidence on the involvement of the brain's serotonergic system in the regulation of food intake, digestion, and metabolism in fish. The last two chapters describe the identification and purification of a novel serotonin-modulating anti-consolidation protein and show how this protein is involved in memory formation and suppression of drug addiction and epileptic seizures in Chapter 6 and in regulation of mutagenesis and adaptation in Chapter 7. Collectively, this book provides a broad view of recent advances in serotonin research in different species of animals. It is our hope that the book will provide readers with a deeper understanding of these new developments in serotonin research across different disciplines.

The primary objective of this book is to provide updated knowledge of recent advances in proteomics research in various fields. The topics covered include, but are not limited to, proteomic analysis for microbiomes, specific cell types from heterogeneous tissues, body fluids, mitochondria, and environmental toxicology. Almost all proteomics techniques currently used in research have been reviewed in at least one chapter, including traditional one- or two-dimensional gel-based, gel-free shotgun, label-based or labelfree quantitative, and array-based methods. In addition, although they are not the major focus of this book, proteomics-related bioinformatics tools such as database searching, data mining, data analysis, and gene pathway analysis are briefly covered. Additionally, this book contains chapters on some technical issues related to applying proteomics technologies to specific issues, such as sample preparation for removal of contaminants and depletion of highly abundant proteins. Collectively, these chapters provide a broad view of various technologies commonly used in proteomics research and their successful applications to address different questions across various species. It is our hope that such a book can provide readers with a comprehensive understanding of proteomics technologies and their application to address various scientific questions.