This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis, as well as data processing and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry for Metabolomics serves as a timely guide for chemists, biochemists, biologists, nutritionists, clinicians, and other experts working in the growing and exciting field of metabolomics.

A changing planet means new problems - and new friends - for nuestros amigos favoritos! Meet Lupe, a whip-smart impala with a flair for mechanics Flapjack, a sweet young octopus who can shine up anything with his eight gleaming tentacles and Elirio, a thoughtful mosquito who's fascinated with words and determined to become an artist. What do all three have in common? A love of lowriders - and a passion for solving problems! Nothing is normal in the little town where the Lowriders live. To start, Flappy can't see a thing! He keeps mistaking fire hydrants for sailors and laundry for love interests. Even more worrying, the Upscale Business Association is determined to make more money than ever by tearing down local shops in favor of a brand-new development for wealthy landowners. Most disconcerting of all, the monarchs who usually migrate through town at this time of year are nowhere to be found. But when Sokar, a beautiful young monarch, bikes into town with a broken wing, she has scary news to report: A dangerous wildfire is burning fast and hot and nonstop, leaving the monarchs stranded. Might Sokar and Flappy have more in common than meets the goggles? How can the Lowriders save their town? And exactly how powerful is passion in the face of an overheated planet's furious flames? Humor, Spanish, and lowriders come together in this rollicking journey through the bumpy terrain of new friends, climate change, and standing up for what you believe in. !Vamonos!