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Vincennes (Hardcover)
Richard Day, William Hopper; Created by Arcadia Publishing
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R662
Discovery Miles 6 620
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Ships in 12 - 17 working days
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The detection and measurement of the dynamic interactions of
proteins within the living cell are critical to our understanding
of cell physiology and pathophysiology. With FRET microscopy and
spectroscopy techniques, basic and clinical scientists can make
such measurements at very high spatial and temporal resolution. But
sources of background information about these tools are very
limited, so this book fills an important gap. It covers both the
basic concepts and theory behind the various FRET microscopy and
spectroscopy techniques, and the practical aspects of using the
techniques and analyzing the results. The critical tricks for
obtaining a good FRET image and precisely quantitating the signals
from living specimens at the nanomolecular level are explained.
Valuable information about the preparation of biological samples
used for FRET image analysis is also provided.
The methods covered include different types of microscopy systems
and detectors (wide-field, confocal, multi-photon) as well as
specialized techniques such as photobleaching FRET, FLIM-FRET
microscopy, spectral imaging FRET, single molecule FRET, and time
and image correlation spectroscopy. Starting from the basics, the
chapters guide readers through the choice of probes to be used for
FRET experiments and the selection of the most suitable
experimental approaches to address specific biological questions.
Up-to-date, consistently organized, and well-illustrated, this
unique book will be welcomed by all researchers who wish to use
FRET microscopy and spectroscopy techniques.
Church History, V7, No. 4, December, 1938.
Proceedings of the Second International Conference on Advanced
Composite Materials and Technologies for Aerospace Applications
held at Glynd
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