This edited volume discusses the application of very diverse human organotypic models in major areas of biomedical research. The authors lay a main focus on infectious diseases, cancer, allergies, as well as drug/vaccine discovery and toxicology studies. Representing a valid alternative to laboratory animals, these models are relevant for most areas of translational research. As the contemporary research shows, many human tissues can today be cultivated in vitro and used for several research objectives. This book provides an unprecedented overview of recent developments in an exciting field of research methodology. It is a reference guide for scientists in both academia and industry. Readers can update their knowledge and get hands-on recommendations on how to set up an organotypic model in their lab. Chapters 'Progress on Reconstructed Human Skin Models for Allergy Research and Identifying Contact Sensitizers' and 'Human Organotypic Models for Anti-infective Research' of this book are available open access under a CC BY 4.0 license at link.springer.com.

This edited volume discusses the application of very diverse human organotypic models in major areas of biomedical research. The authors lay a main focus on infectious diseases, cancer, allergies, as well as drug/vaccine discovery and toxicology studies. Representing a valid alternative to laboratory animals, these models are relevant for most areas of translational research. As the contemporary research shows, many human tissues can today be cultivated in vitro and used for several research objectives. This book provides an unprecedented overview of recent developments in an exciting field of research methodology. It is a reference guide for scientists in both academia and industry. Readers can update their knowledge and get hands-on recommendations on how to set up an organotypic model in their lab. Chapters 'Progress on Reconstructed Human Skin Models for Allergy Research and Identifying Contact Sensitizers' and 'Human Organotypic Models for Anti-infective Research' of this book are available open access under a CC BY 4.0 license at link.springer.com.

This volume offers a comprehensive overview of basic and applied aspects of Staphylococcus aureus, which is one of the most important human pathogens. It includes sixteen chapters that address the microbiology and immunology of S. aureus, the pathology of its key manifestations, and the current standard of care. Further, it reviews cutting-edge advances in alternative therapeutic and prophylactic approaches to antibiotics. All chapters were written by respected experts in the field - presenting recent findings on a diverse range of aspects, they are nonetheless interlinked. As such, the book is a must-read for all researchers, clinicians and technicians engaged in basic or applied science work involving S. aureus.

This book focuses on the envelope of Gram-positive bacteria including its composition, the latest discoveries in the mechanisms behind its assembly, and its role in pathogenesis. Furthermore, new applications in biotechnology and vaccine development involving these bacteria are discussed in detail. This concise volume consists of eleven chapters by prominent experts in the field, which review the latest findings and current state of knowledge on a range of diverse yet interlinked aspects. This book is written for all researchers, clinicians and technicians engaged in basic or applied science projects on Gram-positive bacteria.

This book focuses on the envelope of Gram-positive bacteria including its composition, the latest discoveries in the mechanisms behind its assembly, and its role in pathogenesis. Furthermore, new applications in biotechnology and vaccine development involving these bacteria are discussed in detail. This concise volume consists of eleven chapters by prominent experts in the field, which review the latest findings and current state of knowledge on a range of diverse yet interlinked aspects. This book is written for all researchers, clinicians and technicians engaged in basic or applied science projects on Gram-positive bacteria.

In order to survive, bacteria must constantly monitor their structure and physiology. Adaptive behaviours are established by using environmental sensors and response regulators. These communication modules regulate a wide variety of signals including host detection and invasion, cell cycle, metabolite utilization, starvation and many others. The purpose of this volume is to give an overview of the various systems and to introduce recent advances in understanding selected systems of pathogenic bacteria.

Technological advances, together with a better understanding of the molecular biology of infectious microorganisms, are creating exciting possibilities for a new generation of replicating vaccines. Historically, live vaccines have been either directly derived from a natural source or attenuated by empirical approaches using serial passages and host cell adaptation. Currently, we are witnessing a quantum leap in our technological capabilities to specifically modify the genetic make-up of viruses and bacteria, making it possible to generate improved live vaccines and to develop completely new types of replicating vaccines, such as vectored vaccines, single-round infectious vaccines and replicon vaccines. This book highlights some of the most exciting recent developments towards a new generation of replicating vaccines.

The emergence of H5N1 avian influenza in 1997 and of the influenza A H1N1 of swine origin in 2009 calls for new, rapid and sustainable solutions for both seasonal and pandemic influenza viruses. During the last ten years, science and technology have made enormous progress, and we are now able to monitor in real time the genetics of viruses while they spread globally, to make more powerful vaccines using novel adjuvants, and to generate viruses in the laboratory using reverse genetics. This volume not only provides state-of-the-art information on the biology of influenza viruses and on influenza vaccines, but is also designed to be a resource to face the present H1N1 pandemic and to plan for long-term global and sustainable solutions.

The emergence of H5N1 avian influenza in 1997 and of the influenza A H1N1 of swine origin in 2009 calls for new, rapid and sustainable solutions for both seasonal and pandemic influenza viruses. During the last ten years, science and technology have made enormous progress, and we are now able to monitor in real time the genetics of viruses while they spread globally, to make more powerful vaccines using novel adjuvants, and to generate viruses in the laboratory using reverse genetics. This volume not only provides state-of-the-art information on the biology of influenza viruses and on influenza vaccines, but is also designed to be a resource to face the present H1N1 pandemic and to plan for long-term global and sustainable solutions.

Technological advances, together with a better understanding of the molecular biology of infectious microorganisms, are creating exciting possibilities for a new generation of replicating vaccines. Historically, live vaccines have been either directly derived from a natural source or attenuated by empirical approaches using serial passages and host cell adaptation. Currently, we are witnessing a quantum leap in our technological capabilities to specifically modify the genetic make-up of viruses and bacteria, making it possible to generate improved live vaccines and to develop completely new types of replicating vaccines, such as vectored vaccines, single-round infectious vaccines and replicon vaccines. This book highlights some of the most exciting recent developments towards a new generation of replicating vaccines.

Highlighting the latest activities and initiatives of prominent organizations working in the vaccine industry such as the Bill and Melinda Gates Foundation, The Global Alliance for Vaccines and Immunization, WHO, UNICEF, the World Bank, New Generation Vaccines, Fourth Edition, details steps developing countries have taken toward research, development, manufacture, and regulation of several new vaccines for widespread use.

This text will:

• cover the current state-of-the-art techniques in vaccine development a " including the successes and the failures
• trace vaccine development from the bench to public health with regard to both FDA and European Union regulations
• investigate improved methods for immunizing large populations, and the use of needless vaccinations
• discuss the advancements in the heavily government-funded areas for developing vaccines against potential bioterror and infectious disease agents as well as the immunization of large population bases for diseases like: Anthrax, Smallpox, Ebola, West Nile, SARS, and others

Updated throughout with new cutting-edge information on recent breakthroughs and developments.

NEW TO GENERATION VACCINES, FOURTH EDITION:

• highlights the latest activities of prominent organizations in the vaccine industry
• covers the current techniques in vaccine development
• investigates improved methods for immunizing large populations

Highlighting the latest activities and initiatives of prominent organizations working in the vaccine industry such as the Bill and Melinda Gates Foundation, The Global Alliance for Vaccines and Immunization, WHO, UNICEF, the World Bank, New Generation Vaccines, Fourth Edition, details steps developing countries have taken toward research, development, manufacture, and regulation of several new vaccines for widespread use. This text will: cover the current state-of-the-art techniques in vaccine development - including the successes and the failures trace vaccine development from the bench to public health with regard to both FDA and European Union regulations investigate improved methods for immunizing large populations, and the use of needless vaccinations discuss the advancements in the heavily government-funded areas for developing vaccines against potential bioterror and infectious disease agents as well as the immunization of large population bases for diseases like: Anthrax, Smallpox, Ebola, West Nile, SARS, and others Updated throughout with new cutting-edge information on recent breakthroughs and developments. NEW TO GENERATION VACCINES, FOURTH EDITION: highlights the latest activities of prominent organizations in the vaccine industry covers the current techniques in vaccine development investigates improved methods for immunizing large populations

A new coronavirus, most likely spilled over from an animal species, has plunged us into the third epidemic of this kind in the last twenty years, against which there were neither vaccines nor therapies.While we argue over the future of humanity, vulnerable to the ecological and environmental degradation that has enabled the pandemic, extraordinary technologies have been developed to combat infectious diseases. In just eleven months it was possible to develop, test and produce the vaccines that are gradually enabling us to escape the SARS-CoV-2 nightmare. In addition, with the legacy of the technologies developed against COVID-19, we will be able to overcome antimicrobial resistance-a slow but inexorable pandemic.As vaccinologists are churning out increasingly precise and effective solutions, vaccine acceptance seems to be receding. Outbreaks of preventable diseases have prompted the health authorities of several countries to make childhood vaccinations mandatory again. Much remains to be done, but a public capable of distinguishing authoritative voices from misleading ones will be able to enjoy the vaccines of tomorrow more widely. Vaccines in the Global Era is an easy-to-read book that can be read by virtually anyone who wants to learn about the importance, effectiveness and safety of vaccines in preventing infectious diseases.Vaccines are cheap, save countless lives, and are more effective than the best medicines. Let's try to make the best use of them for the health of the people and animals living together on this beautiful planet.

This volume offers a comprehensive overview of basic and applied aspects of Staphylococcus aureus, which is one of the most important human pathogens. It includes sixteen chapters that address the microbiology and immunology of S. aureus, the pathology of its key manifestations, and the current standard of care. Further, it reviews cutting-edge advances in alternative therapeutic and prophylactic approaches to antibiotics. All chapters were written by respected experts in the field - presenting recent findings on a diverse range of aspects, they are nonetheless interlinked. As such, the book is a must-read for all researchers, clinicians and technicians engaged in basic or applied science work involving S. aureus.

A new coronavirus, most likely spilled over from an animal species, has plunged us into the third epidemic of this kind in the last twenty years, against which there were neither vaccines nor therapies.While we argue over the future of humanity, vulnerable to the ecological and environmental degradation that has enabled the pandemic, extraordinary technologies have been developed to combat infectious diseases. In just eleven months it was possible to develop, test and produce the vaccines that are gradually enabling us to escape the SARS-CoV-2 nightmare. In addition, with the legacy of the technologies developed against COVID-19, we will be able to overcome antimicrobial resistance-a slow but inexorable pandemic.As vaccinologists are churning out increasingly precise and effective solutions, vaccine acceptance seems to be receding. Outbreaks of preventable diseases have prompted the health authorities of several countries to make childhood vaccinations mandatory again. Much remains to be done, but a public capable of distinguishing authoritative voices from misleading ones will be able to enjoy the vaccines of tomorrow more widely. Vaccines in the Global Era is an easy-to-read book that can be read by virtually anyone who wants to learn about the importance, effectiveness and safety of vaccines in preventing infectious diseases.Vaccines are cheap, save countless lives, and are more effective than the best medicines. Let's try to make the best use of them for the health of the people and animals living together on this beautiful planet.

Vaccines have long been used to combat infectious diseases, however the last decade has witnessed a revolution in the approach to vaccine design and development. No longer is there a need to rely on the laborious classical methods, such as attenuation or killing the pathogen. Now, sophisticated technologies - such as genomics, proteomics, functional genomics, and synthetic chemistry - can be used for the rational identification of antigens, the synthesis of complex glycans, the generation of engineered carrier proteins, and much more. Never has research in this area been more exciting. In this book, expert international contributors critically review the current cutting-edge research in vaccine design and development. Particular emphasis is given to new approaches and technologies. The book has been divided into two parts. The first part reviews the technologies and approaches used to identify, generate, and test new vaccines. Topics include new strategies to identify protective antigens, generation of improved adjuvants, use of alternative immunization routes, improving vaccine safety, and finding and establishing the correlates of protection. The second part of the book focuses on the development of new vaccines to replace or complement currently available products or for diseases against which prophylactic strategies are missing. Examples include vaccines against nosocomial infections, streptococci, emerging viral diseases, P. aeruginosa, and bovine mastitis.

SMART Vaccines - Strategic Multi-Attribute Ranking Tool for Vaccines - is a prioritization software tool developed by the Institute of Medicine that utilizes decision science and modeling to help inform choices among candidates for new vaccine development. A blueprint for this computer-based guide was presented in the 2012 report Ranking Vaccines: A Prioritization Framework: Phase I. The 2013 Phase II report refined a beta version of the model developed in the Phase I report. Ranking Vaccines: Applications of a Prioritization Software Tool: Phase III: Use Case Studies and Data Framework extends this project by demonstrating the practical applications of SMART Vaccines through use case scenarios in partnership with the Public Health Agency of Canada, New York State Department of Health, and the Serum Institute of India. This report also explores a novel application of SMART Vaccines in determining new vaccine product profiles, and offers practical strategies for data synthesis and estimation to encourage the broader use of the software. Table of Contents Front Matter Summary 1 Introduction: SMART Vaccines and Smart Priorities 2 Data Synthesis and Framework 3 Use Case Scenarios and Design Enhancements 4 Reflections and Looking Forward References Appendix A: Use Case Scenarios Report for SMART Vaccines Appendix B: Committee's Response to the Use Case Scenarios Report Appendix C: SMART Vaccines Software Updates Appendix D: Stakeholder Speakers Appendix E: Biographical Information

SMART Vaccines-Strategic Multi-Attribute Ranking Tool for Vaccines-is a prioritization software tool developed by the Institute of Medicine that utilizes decision science and modeling to help inform choices among candidates for new vaccine development. A blueprint for this computer-based guide was presented in the 2012 report Ranking Vaccines: A Prioritization Framework: Phase I. Ranking Vaccines: A Prioritization Software Tool,Phase II extends the proof-of-concept presented in the Phase I report, which was based on multi-attribute utility theory. This report refines a beta version of the model developed in the Phase I report and presents its next iteration, SMART Vaccines 1.0. Ranking Vaccines: Phase II discusses the methods underlying the development, validation, and evaluation of SMART Vaccines 1.0. It also discusses how SMART Vaccines should-and, just as importantly, should not-be used. The report also offers ideas for future enhancements for SMART Vaccines as well as for ideas for expanded uses and considerations and possibilities for the future. Table of Contents Front Matter Summary 1 Introduction: New Vaccines and SMART Vaccines 2 Refinements to the SMART Vaccines Model 3 Data Synthesis, Software Redesign, and Evaluation 4 Observations and Looking Forward References Appendix A: Computational Modeling for SMART Vaccines Appendix B: Candidate Disease Profiles Appendix C: Data Sources and Methodology for SMART Vaccines Appendix D: Verification and Analyses of the SMART Vaccines Computational Model Appendix E: Stakeholder Speakers Appendix F: Biographical Information